AMPK β subunits display isoform specific affinities for carbohydrates

被引：53

作者：

Koay, Ann ^{[1
]}

Woodcroft, Ben ^{[1
]}

Petrie, Emma J. ^{[1
]}

Yue, Helen ^{[1
]}

Emanuelle, Shane ^{[1
]}

Bieri, Michael ^{[1
]}

Bailey, Michael F. ^{[1
]}

Hargreaves, Mark ^{[2
]}

Park, Jong-Tae ^{[3
,4
]}

Park, Kwan-Hwa ^{[3
,4
]}

Ralph, Stuart ^{[1
]}

Neumann, Dietbert ^{[5
]}

Stapleton, David ^{[1
]}

Gooley, Paul R. ^{[1
]}

机构：

[1] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Parkville, Vic 3052, Australia

[2] Univ Melbourne, Dept Physiol, Parkville, Vic 3052, Australia

[3] Univ Incheon, Ctr Agr Biomat, Inchon 402749, South Korea

[4] Univ Incheon, Dept Biol, Inchon 402749, South Korea

[5] ETH, Dept Biol, Inst Cell Biol, CH-8093 Zurich, Switzerland

来源：

FEBS LETTERS | 2010年 / 584卷 / 15期

基金：

瑞士国家科学基金会; 澳大利亚研究理事会; 英国医学研究理事会;

关键词：

AMP-activated protein kinase; beta-Subunit; Carbohydrate-binding module; Glycogen; Oligosaccharide; ACTIVATED PROTEIN-KINASE; HUMAN SKELETAL-MUSCLE; BINDING MODULE; GLYCOGEN; RECOGNITION; DOMAIN;

D O I：

10.1016/j.febslet.2010.07.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

AMP-activated protein kinase (AMPK) is a heterotrimer of catalytic (alpha) and regulatory (beta and gamma) subunits with at least two isoforms for each subunit. AMPK w beta 1 is widely expressed whilst AMPK beta 2 is highly expressed in muscle and both beta isoforms contain a mid-molecule carbohydrate-binding module (beta-CBM). Here we show that beta 2-CBM has evolved to contain a Thr insertion and increased affinity for glycogen mimetics with a preference for oligosaccharides containing a single alpha-1,6 branched residue. Deletion of Thr-101 reduces affinity for single alpha-1,6 branched oligosaccharides by 3-fold, while insertion of this residue into the equivalent position in the beta 1-CBM sequence increases affinity by 3-fold, confirming the functional importance of this residue. (C) 2010 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：3499 / 3503

页数：5

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