High speed spectral imager for imaging transient fluorescence phenomena

被引:1
|
作者
Descour, MR [1 ]
Ford, BK [1 ]
Wilson, DW [1 ]
Maker, PD [1 ]
Bearman, GH [1 ]
机构
[1] Univ Arizona, Ctr Opt Sci, Tucson, AZ 85721 USA
关键词
spectral imaging; high-speed imaging; fluorescence spectroscopy; imaging spectrometry;
D O I
10.1117/12.307327
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We describe fluorescence spectral-imaging results with the microscope computed-tomography imaging spectrometer (mu CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, mu CTIS can be used to image dynamic phenomena. The results presented in this paper consist of imaging results using static targets consisting of 1 mu m and 6 mu m fluorescing microspheres. The emission spectra were sampled at a 10-nm interval between 430 nm and 710 nm. The smallest spatial sampling interval presented in this paper is 1.7 mu m. Image data were collected in integration times of 16 msec.
引用
收藏
页码:11 / 17
页数:7
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