High speed spectral imager for imaging transient fluorescence phenomena

We describe fluorescence spectral-imaging results with the microscope computed-tomography imaging spectrometer (mu CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, mu CTIS can be used to image dynamic phenomena. The results presented in this paper consist of imaging results using static targets consisting of 1 mu m and 6 mu m fluorescing microspheres. The emission spectra were sampled at a 10-nm interval between 430 nm and 710 nm. The smallest spatial sampling interval presented in this paper is 1.7 mu m. Image data were collected in integration times of 16 msec.