Direct screening of libraries of yeast clones for α-amylase activity on raw starch hydrolysis

被引：9
|
作者：
Wong, DWS [1 ]
Batt, SB [1 ]
Lee, CC [1 ]
Robertson, GH [1 ]
机构：
[1] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA
来源：
PROTEIN AND PEPTIDE LETTERS | 2003年 / 10卷 / 05期
关键词：
AMY1; barley seed alpha-amylase; alpha-amylase; starch hydrolysis; yeast library; high-throughput assay;
D O I：
10.2174/0929866033478744
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
High-throughput screening for high-activity barley alpha-amylase mutants expressed in Saccharomyces cerevisiae is hampered by the interference of reducing agents, particularly the glucose used in yeast growth media. The present investigation employed colorimetric and chemiluminescent detection systems that enable direct and rapid screening of activities on raw starch substrate. Active clones could be separated into two groups, based on high total activity or high specific activity.
引用
收藏
页码：459 / 468
页数：10
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