Use of conventional or non-conventional treatments of biochar for lipase immobilization

被引:28
|
作者
Almeida, Lays C. [1 ]
Barbosa, Anderson S. [1 ]
Fricks, Alini T. [1 ]
Freitas, Lisiane S. [2 ]
Lima, Alvaro S. [1 ]
Soares, Cleide M. F. [1 ]
机构
[1] Univ Tiradentes, Inst Technol & Res, Ave Murilo Dantas 300, BR-49032490 Aracaju, Sergipe, Brazil
[2] Univ Fed Sergipe, Dept Chem, Av Marechal Rondon,S-N, BR-49100000 Sao Cristovelo, Sergipe, Brazil
关键词
Biochar; Conventional treatment (muffle) and non-conventional (ultrasound; microwave); Immobilization; Lipase; MESOPOROUS ACTIVATED CARBON; K2CO3; ACTIVATION; BIOCATALYSIS; ADSORPTION; RESIDUE; STRAW; PEEL; OIL; KOH;
D O I
10.1016/j.procbio.2017.06.020
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The objective of this work was to evaluate the influence of dichloromethane (CH2Cl2), potassium hydroxide (KOH) and phosphoric acid (H3PO4) in the treatment of biochar from guava seeds on conventional and non conventional techniques (ultrasound and microwave) for the immobilization of Burkholderia cepacia lipase (BCL) by physical adsorption. The effects of the different treatments on the physical and chemical properties of the biochar were evaluated by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The immobilization of the BCL on the biochar was evaluated in hydrolysis reactions of olive oil. The conventional treatment of the biochar with KOH showed increased modification on the surface of the biochar, which presented a highly porous surface, and a greater activity for the immobilized biocatalyst compared with the other treatments. The results revealed the potential of biochar as a support novelty for the immobilization of enzymes and for their application in biocatalysis.
引用
收藏
页码:124 / 129
页数:6
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