A Novel Amperometric Biosensor Based on Banana Peel (Musa cavendish) Tissue Homogenate for Determination of Phenolic Compounds

被引：9

作者：

Ozcan, Hakki Mevlut ^{[1
]}

Sagiroglu, Ayten ^{[1
]}

机构：

[1] Trakya Univ, Fac Sci, Dept Chem, Biochem Sect, Edirne, Turkey

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2010年 / 38卷 / 04期

关键词：

tissue biosensor; phenolic compound; amperometric biosensor; Musa cavedish; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; POLYPHENOL OXIDASE; PEROXIDASE; FILM;

D O I：

10.3109/10731191003776744

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study the biosensor was constructed by immobilizing tissue homogenate of banana peel onto a glassy carbon electrode surface. Effects of immobilization materials amounts, effects of pH, buffer concentration and temperature on biosensor response were studied. In addition, the detection ranges of 13 phenolic compounds were obtained with the help of the calibration graphs. Storage stability, repeatability of the biosensor, inhibitory effect and sample applications were also investigated. A typical calibration curve for the sensor revealed a linear range of 10-80 mu M catechol. In reproducibility studies, variation coefficient and standard deviation were calculated as 2.69%, 1.44 x 10(-3) mu M, respectively.

引用

页码：208 / 214

页数：7

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