TcMYB8, a R3-MYB Transcription Factor, Positively Regulates Pyrethrin Biosynthesis in Tanacetum cinerariifolium

被引:11
|
作者
Zhou, Li [1 ]
Li, Jiawen [1 ]
Zeng, Tuo [1 ,2 ]
Xu, Zhizhuo [1 ]
Luo, Jing [1 ]
Zheng, Riru [1 ]
Wang, Yuanyuan [1 ]
Wang, Caiyun [1 ]
机构
[1] Huazhong Agr Univ, Coll Hort & Forestry Sci, Key Lab Biol Hort Plants, Minist Educ, Wuhan 430070, Peoples R China
[2] Guizhou Normal Univ, Sch Life Sci, Guiyang 550025, Peoples R China
基金
中国国家自然科学基金;
关键词
function analysis; MYB transcription factors; pyrethrins; Tanacetum cinerariifolium; CHRYSANTHEMYL DIPHOSPHATE SYNTHASE; ARTEMISININ BIOSYNTHESIS; ACCUMULATION; INSECTICIDES; INITIATION; MOIETY; ENZYME; GENE;
D O I
10.3390/ijms232012186
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pyrethrins are a mixture of terpenes, with insecticidal properties, that accumulate in the aboveground parts of the pyrethrum (Tanacetum cinerariifolium). Numerous studies have been published on the positive role of MYB transcription factors (TFs) in terpenoid biosynthesis; however, the role of MYB TFs in pyrethrin biosynthesis remains unknown. Here, we report the isolation and characterization of a T. cinerariifolium MYB gene encoding a R3-MYB protein, TcMYB8, containing a large number of hormone-responsive elements in its promoter. The expression of the TcMYB8 gene showed a downward trend during the development stage of flowers and leaves, and was induced by methyl jasmonate (MeJA), salicylic acid (SA), and abscisic acid (ABA). Transient overexpression of TcMYB8 enhanced the expression of key enzyme-encoding genes, TcCHS and TcGLIP, and increased the content of pyrethrins. By contrast, transient silencing of TcMYB8 decreased pyrethrin contents and downregulated TcCHS and TcGLIP expression. Further analysis indicated that TcMYB8 directly binds to cis-elements in proTcCHS and proTcGLIP to activate their expression, thus regulating pyrethrin biosynthesis. Together, these results highlight the potential application of TcMYB8 for improving the T. cinerariifolium germplasm, and provide insight into the pyrethrin biosynthesis regulation network.
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页数:13
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