A dynamic microarray device for paired bead-based analysis

被引：51
|
作者：
Teshima, Tetsuhiko [1 ]
Ishihara, Hirotaka [1 ]
Iwai, Kosuke [1 ]
Adachi, Aki [1 ]
Takeuchi, Shoji [1 ,2 ]
机构：
[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo, Japan
[2] Kanagawa Acad Sci & Technol, Bio Micro Syst Project, Takatsu Ku, Kawasaki, Kanagawa, Japan
来源：
LAB ON A CHIP | 2010年 / 10卷 / 18期
基金：
日本科学技术振兴机构;
关键词：
MICROFLUIDIC DEVICE; CELL-MEMBRANE; FUSION; SYSTEM; ARRAYS; ELECTROFUSION; ENCAPSULATION; RELEASE; FLOW;
D O I：
10.1039/c004986g
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we have developed a meander-shaped dynamic microfluidic technology that allows us to pair two different types of microbeads in a trapping site. The dynamic microfluidic technology comprises implemented modifications of a conventional dynamic microarray design such as: (i) the combination of a meander-shaped by-pass channel and a trapping channel with a hydrodynamic trapping site and (ii) line-symmetrical formation of the by-pass and trapping channels. Using these modifications, we have successfully trapped different types of sample in one trapping site, and constructed an array of paired beads of different type such as polystyrene beads or hydrogel beads made of agarose, collagen or alginate. We found that this meander-shaped dynamic microfluidic technology is applicable for the observation of interactions between the paired beads such as molecular diffusion.
引用
收藏
页码：2443 / 2448
页数：6
相关论文
共 50 条
  • [21] Bead-Based Extracellular Vesicle Analysis Using Flow Cytometry
    Yang, Katherine S.
    Lin, Hsing-Ying
    Curley, Caleigh
    Welch, Marisa W.
    Wolpin, Brian M.
    Lee, Hakho
    Weissleder, Ralph
    Im, Hyungsoon
    Castro, Cesar M.
    ADVANCED BIOSYSTEMS, 2020, 4 (12)
  • [22] Magnetic bead-based sample preparation
    Lamberg, Arja
    GENETIC ENGINEERING & BIOTECHNOLOGY NEWS, 2007, 27 (12): : 26 - 27
  • [23] Bead-based immunoassays with microelectrode detection
    Svetlana Farrell
    Niina J. Ronkainen-Matsuno
    H. Brian Halsall
    William R. Heineman
    Analytical and Bioanalytical Chemistry, 2004, 379 : 358 - 367
  • [24] The genesis and evolution of bead-based multiplexing
    Graham, Hilary
    Chandler, Don J.
    Dunbar, Sherry A.
    METHODS, 2019, 158 : 2 - 11
  • [25] Single bead-based electrochemical biosensor
    Liu, Changchun
    Schrlau, Michael G.
    Bau, Haim H.
    BIOSENSORS & BIOELECTRONICS, 2009, 25 (04): : 809 - 814
  • [26] An integrated model for bead-based immunoassays
    Wu, Dan
    Voldman, Joel
    BIOSENSORS & BIOELECTRONICS, 2020, 154
  • [27] Bead-based immunoassays with microelectrode detection
    Farrell, S
    Ronkainen-Matsuno, NJ
    Halsall, HB
    Heineman, WR
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2004, 379 (03) : 358 - 367
  • [28] Advances in Bead-Based Biomarker Detection
    McFarlin, Brian K.
    METHODS, 2019, 158 : 1 - 1
  • [29] Fabrication of microfluidic devices incorporating bead-based reaction and microarray-based detection system for enzymatic assay
    Kim, Dae Nyun
    Lee, Yeol
    Koh, Won-Gun
    SENSORS AND ACTUATORS B-CHEMICAL, 2009, 137 (01) : 305 - 312
  • [30] Development of Group B Streptococcus Multiplex PCR Panel Amendable to Bead-Based and Microarray Detection Systems
    Malone, L.
    Brzezinski, S.
    Hockman, D.
    Sesler, C.
    Grigorenko, E.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2013, 15 (06): : 895 - 895
12345