A simple technique for consistently obtaining large single crystals of hen egg-white lysozyme in a concentration gradient of NiCl2

被引：4
|
作者：
Magay, Elena [1 ]
Yoon, Tae-Sung [1 ,2 ]
机构：
[1] Korea Res Inst Biosci & Biotechnol, Med Preote Res Ctr, Taejon, South Korea
[2] Univ Sci & Technol, Taejon, South Korea
来源：
JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2011年 / 44卷
关键词：
hen egg-white lysozyme; crystallization; neutron diffraction; CRYSTALLOGRAPHY;
D O I：
10.1107/S0021889810044936
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A simple protocol is reported for consistently obtaining large single crystals of hen egg-white lysozyme (HEWL) greater than 2 mm in the longest dimension. Comparative crystallization experiments with different sources of HEWL showed that the simple addition of glass capillaries, `crystal hangers', reduced excessive nucleation and resulted in large single crystals regardless of the commercial source of HEWL.
引用
收藏
页码：252 / 253
页数：2
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