Fabricating Gradient Hydrogel Scaffolds for 3D Cell Culture

被引：0

作者：

Chatterjee, Kaushik ^{[1
,2
]}

Young, Marian F. ^{[2
]}

Simon, Carl G., Jr. ^{[1
]}

机构：

[1] NIST, Div Polymers, Gaithersburg, MD 20899 USA

[2] Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2011年 / 14卷 / 04期

关键词：

Combinatorial methods; high-throughput screening; hydrogels; elastic modulus; gradients; osteoblast; tissue engineering; BIOMATERIALS;

D O I：

10.2174/138620711795222455

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimizing cell-material interactions is critical for maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We have developed a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we provide detailed instructions on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.

引用

页码：227 / 236

页数：10

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