Identification of UDP-glucuronosyltransferase isoforms involved in hepatic and intestinal glucuronidation of phytochemical carvacrol

被引：16
|
作者：
Dong, Rui-Hua [2 ]
Fang, Zhong-Ze [1 ]
Zhu, Liang-Liang [1 ]
Ge, Guang-Bo [1 ]
Yang, Ling [1 ]
Liu, Ze-Yuan [2 ]
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
[2] Acad Mil Med Sci, Affiliated Hosp, Dept Clin Pharmacol, Beijing 100071, Peoples R China
来源：
XENOBIOTICA | 2012年 / 42卷 / 10期
基金：
中国国家自然科学基金;
关键词：
UDP-glucuronosyltransferase; carvacrol; human liver microsomes; human intestinal microsomes; LIVER CYTOCHROME-P450 ENZYMES; IN-VITRO; DRUG-GLUCURONIDATION; ANTIOXIDANT ACTIVITY; NOMENCLATURE UPDATE; GENE SUPERFAMILY; ESSENTIAL OILS; SUBSTRATE; THYMOL; XENOBIOTICS;
D O I：
10.3109/00498254.2012.682614
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
1. Carvacrol (2-methyl-5-(1-methylethyl)-phenol), one of the main components occurring in many essential oils of the family Labiatae, has been widely used in food, spice and pharmaceutical industries. 2. The carvacrol glucuronidation was characterized by human liver microsomes (HLMs), human intestinal microsomes (HIMs) and 12 recombinant UGT (rUGT) isoforms. 3. One metabolite was identified as a mono-glucuronide by liquid chromatography/mass spectrometry with HLMs, HIMs, rUGT1A3, rUGT1A6, rUGT1A7, rUGT1A9 and rUGT2B7. 4. The study with a chemical inhibition, rUGT, and kinetics study demonstrated that rUGT1A9 was the major isozyme responsible for glucuronidation in HLMs, and rUGT1A7 played a major role for glucuronidation in HIMs.
引用
收藏
页码：1009 / 1016
页数：8
相关论文
共 50 条
  • [21] Interindividual variability in acetaminophen glucuronidation by human liver microsomes: Identification of relevant acetaminophen UDP-glucuronosyltransferase isoforms
    Court, MH
    Duan, SX
    Von Moltke, LL
    Greenblatt, DJ
    Patten, CJ
    Miners, JO
    Mackenzie, PI
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2001, 299 (03): : 998 - 1006
  • [22] Hepatic glucuronidation of tetrabromobisphenol A and tetrachlorobisphenol A: interspecies differences in humans and laboratory animals and responsible UDP-glucuronosyltransferase isoforms in humans
    Hanioka, Nobumitsu
    Isobe, Takashi
    Saito, Keita
    Nagaoka, Kenjiro
    Mori, Yoko
    Jinno, Hideto
    Ohkawara, Susumu
    Tanaka-Kagawa, Toshiko
    ARCHIVES OF TOXICOLOGY, 2024, 98 (03) : 837 - 848
  • [23] Hepatic glucuronidation of tetrabromobisphenol A and tetrachlorobisphenol A: interspecies differences in humans and laboratory animals and responsible UDP-glucuronosyltransferase isoforms in humans
    Nobumitsu Hanioka
    Takashi Isobe
    Keita Saito
    Kenjiro Nagaoka
    Yoko Mori
    Hideto Jinno
    Susumu Ohkawara
    Toshiko Tanaka-Kagawa
    Archives of Toxicology, 2024, 98 : 837 - 848
  • [24] Effects of ketoconazole on glucuronidation by UDP-glucuronosyltransferase enzymes
    Yong, WP
    Ramirez, J
    Innocenti, F
    Ratain, MJ
    CLINICAL CANCER RESEARCH, 2005, 11 (18) : 6699 - 6704
  • [25] Identification of human UDP-glucuronosyltransferase enzyme(s) responsible for the glucuronidation of strychnine
    Mao, Yuxi
    Fang, Zhong-Ze
    Ge, Guangbo
    Zhang, Yanyan
    Yang, Ling
    DRUG METABOLISM REVIEWS, 2011, 43 : 67 - 67
  • [26] Identification of the human UDP-glucuronosyltransferase responsible for the glucuronidation of niflumic acid in the liver
    Mano, Yuji
    Usui, Takashi
    Kamimura, Hidetaka
    DRUG METABOLISM REVIEWS, 2006, 38 : 252 - 253
  • [27] Glucuronidation of antiallergic drug, tranilast: Identification of human UDP-glucuronosyltransferase isoforms and effect of its phase I metabolite
    Katoh, Miki
    Matsui, Tomohito
    Yokoi, Tsuyoshi
    DRUG METABOLISM AND DISPOSITION, 2007, 35 (04) : 583 - 589
  • [28] Identification and interspecies characterization of UDP-glucuronosyltransferase isoforms catalyzing acacetin glucuronidation using recombinant UGT enzymes and microsomes
    Kangle Shi
    Shan Li
    Qinggang Meng
    Journal of Traditional Chinese Medical Sciences, 2019, 6 (02) : 155 - 163
  • [29] Hepatic glucuronidation of 4-tert-octylphenol in humans: inter-individual variability and responsible UDP-glucuronosyltransferase isoforms
    Takashi Isobe
    Susumu Ohkawara
    Toshiko Tanaka-Kagawa
    Hideto Jinno
    Nobumitsu Hanioka
    Archives of Toxicology, 2017, 91 : 3543 - 3550
  • [30] Hepatic glucuronidation of 4-tert-octylphenol in humans: inter-individual variability and responsible UDP-glucuronosyltransferase isoforms
    Isobe, Takashi
    Ohkawara, Susumu
    Tanaka-Kagawa, Toshiko
    Jinno, Hideto
    Hanioka, Nobumitsu
    ARCHIVES OF TOXICOLOGY, 2017, 91 (11) : 3543 - 3550
12345