Optimisation of serine protease extraction from mango peel (Mangifera Indica Cv. Chokanan)

This study investigated the possible relationship between the enzyme extraction variables, namely amount of buffer (25-75 ml, X-1), temperature (-20,25 degrees C, X-2) and mixing time (1-3 min, X-3) on total protein (Y-1), total activity (Y-2), specific activity (Y-3), storage stability (Y-4), temperature stability (Y-5) and pH stability (Y-6) of serine protease from mango peel. It was found that there was significant (p < 0.05) fit of the response surface models for all the response variables investigated. There was indication of high coefficient of determination (R-2) values (between 0.954 and 1.000) in the regression models describing variations of the response variables. It was found that there was no significant (p > 0.05) difference between the experimental and predicted values. This ensured that the response surface models used to indicate property changes of serine protease as a function of enzyme extraction conditions were sufficient. (c) 2010 Elsevier Ltd. All rights reserved.