MiR-23a targets RUNX2 and suppresses ginsenoside Rg1-induced angiogenesis in endothelial cells

被引:20
|
作者
Wu, Xiao-Dong [1 ]
Guo, Ting [4 ]
Liu, Li [5 ]
Wang, Chao [5 ]
Zhang, Kun [1 ,6 ]
Liu, Han-Qiang [4 ]
Wang, Feng [4 ]
Bai, Wen-Dong [7 ]
Zhang, Meng-Yao [1 ,2 ,3 ]
机构
[1] Fourth Mil Med Univ, Dept Cell Biol, Natl Translat Sci Ctr Mol Med, Xian 710032, Shaanxi, Peoples R China
[2] Beijing Inst Biotechnol, Beijing 100071, Peoples R China
[3] Fourth Mil Med Univ, Xijing Hosp, Dept Clin Immunol, Xian 710032, Shaanxi, Peoples R China
[4] Fourth Mil Med Univ, Dept Nutr & Food Hyg, Xian 710032, Shaanxi, Peoples R China
[5] Peoples Liberat Army Gen Hosp, Dept Stomatol, Beijing 100700, Peoples R China
[6] Beijing Jiaotong Univ, Sch Sci, Coll Life Sci & Bioengn, Beijing 100044, Peoples R China
[7] PLA, Clin Lab Med Ctr, Xinjiang Command Gen Hosp, Urumqi 830000, Peoples R China
基金
中国博士后科学基金;
关键词
angiogenesis; ginsenoside Rg1; miR-23a; runt-related transcription factor 2; vascular endothelial growth factor; GROWTH-FACTOR; EXPRESSION; PATHWAY; RG1; DIFFERENTIATION; COMMITMENT; MICRORNAS; MECHANISM; RESPONSES; BIOLOGY;
D O I
10.18632/oncotarget.19489
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Rg1 is a predominant protopanaxatriol-type of ginsenoside found in Panax ginseng, and it has been shown to have anti-cancer effects in multiple types of cancer cells. However, Rg1 also induces the expression of proangiogenic factors, such as vascular endothelial growth factor (VEGF-A), in endothelial cells. Unfortunately, angiogenesis positively correlates with cancer development. In this study, we identified RUNX2 as a regulator of ginsenoside Rg1-induced angiogenesis for the first time. We found that RUNX2 was directly targeted and regulated by miR-23a. Additionally, miR-23a was shown to inhibit angiogenesis in both human umbilical vein endothelial cells (HUVECs) and in zebrafish. Furthermore, a decrease in RUNX2 expression resulted in translational repression of VEGF-A in HUVECs. Taken together, this study identified a MiR-23a/RUNX2/VEGF-A pathway in angiogenesis and shed light on the molecular mechanism of Rg1-induced angiogenesis. Thus, RUNX2 might be a potential therapeutic target in Rg1-mediated angiogenesis in cancer.
引用
收藏
页码:58072 / 58085
页数:14
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