A Combination of the Natural Molecules Gallic Acid and Carvacrol Eradicates P. aeruginosa and S. aureus Mature Biofilms

被引:18
|
作者
Gobin, Maxime [1 ]
Proust, Richard [2 ]
Lack, Stephane [2 ]
Duciel, Laura [2 ]
Des Courtils, Celine [2 ]
Pauthe, Emmanuel [1 ]
Gand, Adeline [1 ]
Seyer, Damien [1 ]
机构
[1] CY Cergy Paris Univ, ERRMECe Lab, 1 Rue Descartes, F-95031 Neuville Sur Oise, France
[2] Labs BROTHIER, 41 Rue Neuilly, F-92735 Nanterre, France
关键词
biofilm; wounds; carvacrol; gallic acid; curcumin; combination therapies; PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL ACTIVITY; ESSENTIAL OIL; CURCUMIN; VIRULENCE; OREGANO; IRON;
D O I
10.3390/ijms23137118
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Wound infection, especially the development of bacterial biofilms, delays wound healing and is a major public health concern. Bacteria in biofilms are more tolerant to antimicrobial agents, and new treatments to eradicate mature biofilms are needed. Combining antimicrobial molecules with different mechanisms of action is an attractive strategy to tackle the heterogeneous nature of microbial communities in biofilms. This study focused on three molecules of natural origin: gallic acid (G), carvacrol (K) and curcumin (Q). Their abilities, individually or in combination, to eradicate biofilms were quantified on mono- and dual-species mature biofilms of Pseudomonas aeruginosa and Staphylococcus aureus, the strains most commonly found in infected wounds. G presented biofilm eradicating activity on P. aeruginosa, whereas K had biofilm eradicating activity on S. aureus and P. aeruginosa. Q had no potent biofilm eradicating activity. The combination of G and K increased the effects previously observed on P. aeruginosa biofilm and led to complete eradication of S. aureus biofilm. This combination was also efficient in eradicating a dual-species biofilm of S. aureus and P. aeruginosa. This work demonstrates that K and G used in combination have a strong and synergistic eradicating activity on both mono- and dual-species mature biofilms of S. aureus and P. aeruginosa and may therefore represent an efficient alternative for the treatment of biofilms in wounds.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] THE IMPACT OF P. AERUGINOSA AND S. AUREUS INFECTIONS ON INFLAMMATORY BIOMARKERS IN ADULT CYSTIC FIBROSIS PATIENTS
    Ahlgren, H.
    Matouk, E.
    Landry, J.
    Gruber, J.
    Rousseau, S.
    Benedetti, A.
    Bernier, J.
    Wojewodka, G.
    Lands, L.
    Radzioch, D.
    Nguyen, D.
    PEDIATRIC PULMONOLOGY, 2012, 47 : 330 - 330
  • [22] Mass spectrometric study of antibiotic resistance of S. aureus and P. aeruginosa using the MALDIquant package
    Aleshukina, A.
    Denisenko, V.
    Aleshukina, I.
    Ermakova, L.
    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2022, 116 : S12 - S12
  • [23] From enemies to lovers: The trophic cooperation between S. aureus and P. aeruginosa around acetoin
    Blanc, Jules
    Cadot-Jet, Ninon
    Deshayes, Tom
    M S-MEDECINE SCIENCES, 2023, 39 (04): : 387 - 389
  • [24] Development of stable co-culture of P. aeruginosa and S. aureus in Artificial Sputum Medium
    Vasiljevs, Stanislavs
    Gupta, Arya
    Baines, Deborah
    FASEB JOURNAL, 2021, 35
  • [25] Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections
    Kielholz, Tobias
    Rohde, Felix
    Jung, Nathalie
    Windbergs, Maike
    SCIENTIFIC REPORTS, 2023, 13 (01)
  • [26] Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections
    Tobias Kielholz
    Felix Rohde
    Nathalie Jung
    Maike Windbergs
    Scientific Reports, 13
  • [27] Antibacterial activity of Ocimum sanctum extract against E. coli, S. aureus and P. aeruginosa
    Ghasemi, Elmira
    Golshahi, Hannaneh
    Ghasemi, Elnaz
    Mehranzade, Elham
    CLINICAL BIOCHEMISTRY, 2011, 44 (13) : S352 - S352
  • [28] Distinct Pathogenesis and Host Responses during Infection of C. elegans by P. aeruginosa and S. aureus
    Irazoqui, Javier E.
    Troemel, Emily R.
    Feinbaum, Rhonda L.
    Luhachack, Lyly G.
    Cezairliyan, Brent O.
    Ausubel, Frederick M.
    PLOS PATHOGENS, 2010, 6 (07) : 1 - 24
  • [29] In Silico Comparative Structural and Residue Interaction Network Analysis of MATE Efflux Proteins in P. aeruginosa and S. aureus
    Kumari, Vandna
    Kanwar, Shamsher S.
    CHEMISTRY AFRICA-A JOURNAL OF THE TUNISIAN CHEMICAL SOCIETY, 2022, 5 (05): : 1513 - 1525
  • [30] Niosome-loaded Tet-Amp against S. aureus, K. pneumoniae, and P. aeruginosa
    Bazargan, Elham
    Ashrafi, Fatemeh
    Torbati, Elham Siasi
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2024, 55 (04) : 3563 - 3589