Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells

被引：7
|
作者：
Park, Seong-Hoon [2 ,3 ]
Kang, Hong-Jun [4 ]
Kim, Hyun-Seok [2 ,3 ]
Kim, Min-Ju [5 ]
Heo, Jee-In [1 ,6 ]
Kim, Jeong-Hyeon [6 ]
Kho, Yoon-Jung [1 ]
Kim, Sung Chan [6 ]
Kim, Jaebong [6 ]
Park, Jae-Bong [6 ]
Lee, Jae-Yong [1 ,6 ]
机构：
[1] Hallym Univ, Coll Med, Inst Nat Med, Chunchon 200702, Gangwon Do, South Korea
[2] Vanderbilt Univ, Dept Radiat Oncol, Nashville, TN 37232 USA
[3] Vanderbilt Univ, Dept Pediat, Nashville, TN 37232 USA
[4] NIDDK, Genet Dis Res Sect, NIH, Bethesda, MD 20892 USA
[5] Hallym Univ, Coll Med, Dept Anat & Neurobiol, Chunchon 200702, Gangwon Do, South Korea
[6] Hallym Univ, Coll Med, Dept Biochem, Chunchon 200702, Gangwon Do, South Korea
来源：
BIOGERONTOLOGY | 2011年 / 12卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
DNA damage; DNA repair; DNA repair rate; Mammalian embryonic fibroblasts; Replicative life span; HYDROGEN-PEROXIDE PRODUCTION; MITOCHONDRIAL SUPEROXIDE; TRANSCRIPTION FACTORS; DERMAL FIBROBLASTS; HUMAN-LYMPHOCYTES; OXIDATIVE DAMAGE; POLYMERASE-BETA; EXCISION-REPAIR; IN-VITRO; AGE;
D O I：
10.1007/s10522-011-9355-2
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
Since the detailed comparison of DNA repair activities among mammalian embryonic fibroblast cells with different replicative life spans has not been investigated, we tested DNA repair activities in embryonic fibroblast cells derived from mammals including human, dog, rat, and mouse. The cell viability after treatment of four DNA damage agents appeared to be decreased in the order of human embryonic fibroblasts (HEFs) > dog embryonic fibroblasts (DEFs) > rat embryonic fibroblasts (REFs) > mouse embryonic fibroblasts (MEFs) although statistical significance was lacking. The amounts of strand breaks and AP (apurinic/apyrimidinic) sites also appear to be decreased in the order of HEFs > DEFs > REFs a parts per thousand yen MEFs after treatment of DNA damage agents. The DNA repair activities and rates including base excision repair (BER), nucleotide excision repair (NER) and double-strand break repair (DSBR) including non-homologous end-joining (NHEJ) decreased again in the order of HEFs > DEFs > REFs a parts per thousand yen MEFs. BER and NHEJ activities in 3% O(2) also decreased in the order of HEFs > DEFs > REFs > MEFs. This order in DNA repair activity appears to be coincident with that of replicative life span of fibroblasts and that of life span of mammals. These results indicate that higher DNA repair activity is related with longer replicative life span in embryonic fibroblast cells.
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页码：565 / 579
页数：15
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