Loop-mediated isothermal amplification assays for identification of antiseptic- and methicillin-resistant Staphylococcus aureus

被引：26
|
作者：
Hanaki, Ken-Ichi [2 ]
Sekiguchi, Jun-Ichiro [1 ]
Shimada, Kayo [1 ]
Sato, Ayako [2 ]
Watari, Hajime [3 ]
Kojima, Tadashi [3 ]
Miyoshi-Akiyama, Tohru [1 ]
Kirikae, Teruo [1 ]
机构：
[1] Natl Ctr Global Hlth & Med, Res Inst, Dept Infect Dis, Shinjuku Ku, Tokyo 1628655, Japan
[2] Iwate Med Univ, Ctr Expt Anim Res, Morioka, Iwate 0208505, Japan
[3] Eiken Chem Co Ltd, Biochem Res Lab, Nogi, Tochigi 3290114, Japan
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 84卷 / 02期
关键词：
FemB gene; Loop-mediated isothermal amplification; MecA gene; Methicillin-resistant Staphylococcus aureus; QacA/B gene; QUATERNARY AMMONIUM-COMPOUNDS; POLYMERASE-CHAIN-REACTION; MULTIDRUG-RESISTANCE; DISINFECTANT-RESISTANCE; GENES QACA; MECA; FEMB; PCR; SIMULANS; SMR;
D O I：
10.1016/j.mimet.2010.12.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for rapid identification of antiseptic- and methicillin-resistant Staphylococcus aureus (MRSA) based on 3 loop-mediated isothermal amplification (LAMP) assays was developed. LAMP targeting the femB gene identified S. aureus with 100% specificity, and LAMP targeting the mecA gene associated with methicillin resistance identified methicillin-resistant staphylococci with 100% specificity. LAMP targeting the qacA/B gene encoding an efflux pump responsible for antiseptic resistance identified high-acriflavine-resistant (MIC >= 100 mg/L) MRSA (92.5% positive) and acriflavine-susceptible (MIC < 25 mg/L) MRSA (100% negative). They were performed under the same reaction conditions within 60 min at 63 degrees C. The combined LAMP assays will be useful for rapid identification of S. aureus isolates and determination of their antibiotic and antiseptic resistance patterns with regard to methicillin and organic cationic substrates. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：251 / 254
页数：4
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