Controllable preparation and formation mechanism of BSA microparticles using supercritical assisted atomization with an enhanced mixer

被引:38
|
作者
Wang, Qi [1 ]
Guan, Yi-Xin [1 ]
Yao, Shan-Jing [1 ]
Zhu, Zi-Qiang [1 ]
机构
[1] Zhejiang Univ, Dept Chem & Biol Engn, Hangzhou 310027, Zhejiang, Peoples R China
来源
JOURNAL OF SUPERCRITICAL FLUIDS | 2011年 / 56卷 / 01期
基金
中国国家自然科学基金;
关键词
Supercritical assisted atomization; Hydrodynamic cavitation mixer; Bovine serum albumin; BSA microparticle; Particle formation mechanism; PARTICLE FORMATION; RAPID EXPANSION; NANOPARTICLES; MICRONIZATION; PROTEINS; DELIVERY; ACID;
D O I
10.1016/j.supflu.2010.12.002
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA-HCM) was used to prepare bovine serum albumin (BSA) microparticles. Water was used as the sole solvent. A hydrodynamic cavitation mixer was applied to improve mass transfer and achieve a continuous near-thermodynamic-equilibrium solubilization of SC-CO2 in the liquid solution. Under the different conditions, the prepared BSA microparticles had various morphologies, such as corrugated particles, smooth hollow spherical particles and cup particles, with particle diameters ranging from 0.3 to 5 mu m. The microparticle formation process was elucidated with the shell formation and central bubble mechanism. Compared to native BSA, BSA microparticles did not show significant change in primary structure, according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The secondary structure of BSA was characterized by Fourier transform infrared spectroscopy (FT-IR). No new peaks were observed after SAA-HCM processing. In addition, the crystalline structure of the BSA microparticles was demonstrated to be amorphous because of the sudden supersaturation in the precipitation process. The SAA-HCM process is expected to be a promising technique for producing microparticles suitable for pulmonary delivery of therapeutic macromolecules. Published by Elsevier B.V.
引用
收藏
页码:97 / 104
页数:8
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