LincRNA-p21 exon 1 expression correlates with Cdkn1a expression in vivo

被引：4
|
作者：
Furuhata, Riki [1 ]
Imasaka, Mai [1 ,2 ]
Sugimoto, Michihiko [1 ,3 ]
Yoshinobu, Kumiko [4 ]
Araki, Masatake [4 ]
Araki, Kimi [1 ]
机构：
[1] Kumamoto Univ, Inst Resource Dev & Anal, Div Dev Genet, Kumamoto, Japan
[2] Hyogo Coll Med, Genet, Nishinomiya, Hyogo, Japan
[3] RIKEN, BioResource Res Ctr, Technol & Dev Team Mammalian Genome Dynam, Tsukuba, Ibaraki, Japan
[4] Kumamoto Univ, Inst Resource Dev & Anal, Div Genom, Kumamoto, Japan
来源：
GENES TO CELLS | 2022年 / 27卷 / 01期
基金：
日本学术振兴会;
关键词：
cyclin-dependent kinase inhibitor 1A; enhancer elements; LincRNA; mouse; upregulation; EMBRYONIC STEM-CELLS; GENE-EXPRESSION; CIS; TRANSCRIPTION; ACTIVATION; LNCRNA; RNAS;
D O I：
10.1111/gtc.12906
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
LincRNA-p21 is a long intergenic non-coding RNA (LincRNA) gene reported to activate the transcription of the adjacent Cdkn1a (p21) gene in cis. The importance of the enhancer elements in the LincRNA-p21 gene region has also been reported; however, the involvement of the LincRNA-p21 transcripts in regulating Cdkn1a in vivo is still unclear. In this study, we used a LincRNA-p21-trapped mouse line (LincRNA-p21(Gt)) in which beta geo was inserted into intron 1, and all enhancer elements were retained. In LincRNA-p21(Gt/Gt) mice, the transcription of LincRNA-p21 was repressed due to the beta geo sequence, and the expression of exon 1 of LincRNA-p21 was restored through its deletion or replacement by another sequence, and Cdkn1a expression was also upregulated. Furthermore, regardless of the full-length transcripts, the expression of Cdkn1a correlated with the transcription of the exon 1 of LincRNA-p21. This result indicates that the LincRNA-p21 transcripts are not functional, but the transcriptional activity around exon 1 is important for Cdkn1a expression.
引用
收藏
页码：14 / 24
页数：11
相关论文
共 50 条
  • [41] CDKN1A Gene Expression in Two Multiple Myeloma Cell Lines With Different P53 Functionality
    Drozdkova, Denisa Hrckova
    Gursky, Jan
    Minarik, Jiri
    Uberall, Ivo
    Kolar, Zdenek
    Trtkova, Katerina Smesny
    ANTICANCER RESEARCH, 2020, 40 (09) : 4979 - 4987
  • [42] miR-3619-5p inhibits prostate cancer cell growth by activating CDKN1A expression
    Li, Senmao
    Wang, Chenghe
    Yu, Xiao
    Wu, Huanlei
    Hu, Jia
    Wang, Shaogang
    Ye, Zhangqun
    ONCOLOGY REPORTS, 2017, 37 (01) : 241 - 248
  • [43] Mitotic p21Cip1/CDKN1A is regulated by cyclin-dependent kinase 1 phosphorylation
    Kreis, Nina-Naomi
    Friemel, Alexandra
    Zimmer, Brigitte
    Roth, Susanne
    Rieger, Michael A.
    Rolle, Udo
    Louwen, Frank
    Yuan, Juping
    ONCOTARGET, 2016, 7 (31) : 50215 - 50228
  • [44] LRH-1 controls proliferation in breast tumor cells by regulating CDKN1A gene expression
    S Bianco
    M Jangal
    D Garneau
    N Gévry
    Oncogene, 2015, 34 : 4509 - 4518
  • [45] LRH-1 controls proliferation in breast tumor cells by regulating CDKN1A gene expression
    Bianco, S.
    Jangal, M.
    Garneau, D.
    Gevry, N.
    ONCOGENE, 2015, 34 (34) : 4509 - 4518
  • [46] LincRNA-p21 Inhibits Cell Viability and Promotes Cell Apoptosis in Parkinson's Disease through Activating -Synuclein Expression
    Xu, Xiaonan
    Zhuang, Chengle
    Wu, Zimu
    Qiu, Hongyan
    Feng, Haixia
    Wu, Jun
    BIOMED RESEARCH INTERNATIONAL, 2018, 2018
  • [47] Role of the CDKN1A/p21, CDKN1C/p57, and CDKN2A/p16 genes in the risk of atherosclerosis and myocardial infarction
    Rodriguez, Isabel
    Coto, Eliecer
    Reguero, Julian R.
    Gonzalez, Pelayo
    Andres, Vicente
    Lozano, Inigo
    Martin, Maria
    Alvarez, Victoria
    Moris, Cesar
    CELL CYCLE, 2007, 6 (05) : 620 - 625
  • [48] Development of autoimmune pancreatitis is independent of CDKN1A/p21-mediated pancreatic inflammation
    Seleznik, Gitta M.
    Reding, Theresia
    Peter, Lukas
    Gupta, Anurag
    Steiner, Sabrina G.
    Sonda, Sabrina
    Verbeke, Caroline S.
    Dejardin, Emmanuel
    Khatkov, Igor
    Segerer, Stephan
    Heikenwalder, Mathias
    Graf, Rolf
    GUT, 2018, 67 (09) : 1663 - 1673
  • [49] Inhibition of the miRNA familiy miR-130 restores the expression of CDKN1A (p21) and ameliorates hypoxia-induced pulmonary hypertension
    Brock, Matthias
    Haider, Thomas J.
    Gassmann, Max
    Vogel, Johannes
    Kohler, Malcolm
    Ulrich, Silvia
    Huber, Lars C.
    EUROPEAN RESPIRATORY JOURNAL, 2014, 44
  • [50] CAR-mediated repression of Cdkn1a(p21) is accompanied by the Akt activation
    Yarushkin, Andrei A.
    Mazin, Mark E.
    Yunusova, Anastasia Y.
    Korchagina, Kseniya, V
    Pustylnyak, Yuliya A.
    Prokopyeva, Elena A.
    Pustylnyak, Vladimir O.
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 504 (02) : 361 - 366
12345