Involvement of NF-κB activation in the induction of type II nitric oxide synthase in human glioblastoma cells

被引：1

作者：

Kobayashi, M ^{[1
]}

Suzuki, T ^{[1
]}

Suzuki, I ^{[1
]}

Liou, SY ^{[1
]}

Hashimoto, Y ^{[1
]}

Hashimoto, K ^{[1
]}

机构：

[1] Nippon Glaxo Ltd, Tsukuba Res Labs, Tsukuba, Ibaraki 3004247, Japan

来源：

BIOMEDICAL RESEARCH-TOKYO | 1998年 / 19卷 / 02期

关键词：

D O I：

10.2220/biomedres.19.117

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Nitric oxide (NO) produced by glial cells has been implicated in the pathogenesis of neurodegenerative diseases. In human glial cells, the transcriptional mechanism of inducible NO synthase (iNOS) remains to be understood. We examined the role of the transcription factor NF-xB in the induction of iNOS in a human glioblastoma cell line, A-172. Treatment of A-172 cells with lipopolysaccharide (LPS), interleukin-1 beta (IL-1 beta) and interferon-gamma (IFN-gamma) induced iNOS mRNA and protein expression subsequent to the degradation of I Chi B, a protein that inhibits NF-Chi B, and the DNA binding of NF-Chi B. Furthermore, the antioxidant pyrroridine dithiocarbamate (PDTC), which is known to inhibit NF-Chi B activation, inhibited nitrite production in a dose-dependent manner. The inhibitory effect of PDTC correlated with the prevention of I Chi B degradation. Another antioxidant, nordihydroguaiaretic acid (NDGA), also inhibited the nitrite formation and iNOS mRNA expression through its preventive effect on NF-Chi B activation. These results suggest the involvement of NF-Chi B activation in iNOS induction in human glial cells.

引用

页码：117 / 126

页数：10

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