Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves

被引：6
|
作者：
Saggaf, Maliha H. [1 ]
Ndunguru, Joseph [1 ]
Tairo, Fred [1 ]
Sseruwagi, Peter [1 ]
Ascencio-Ibanez, Jose Trino [3 ]
Kilalo, Dora [2 ]
Miano, Douglas W. [2 ]
机构：
[1] Mikocheni Agr Res Inst, POB 6226, Dar Es Salaam, Tanzania
[2] Univ Nairobi, POB 30197-00100, Nairobi, Kenya
[3] North Carolina State Univ, Raleigh, NC 27695 USA
来源：
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2019年 / 105卷
基金：
比尔及梅琳达.盖茨基金会; 美国国家科学基金会;
关键词：
Manihot esculenta; Cassava brown streak virus (CBSV); Immunohistochemistry; Cassava brown streak disease; Immunolocalization; MOSAIC-VIRUS; GEMINIVIRUS INFECTION; IMMUNOLOCALIZATION; TRANSMISSION; METABOLISM; EXPRESSION; DISEASE; PROTEIN; CARBON; PLANTS;
D O I：
10.1016/j.pmpp.2018.06.001
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The localization of Cassava brown streak virus ( CBSV) in cassava (Manihot esculenta) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed precipitation in CBSV-infected but not CBSV-free tissues, demonstrating successful localization of CBSV. Microscopic inspection showed significantly larger (P < 0.001) midribs in CBSV-infected compared with control (uninfected) leaves. Viral accumulation occurred in middle and lower but rarely in young upper leaves. This immunohistochemical method for virus localization will be invaluable for efficient screening of CBSV and for breeding resistant cassava.
引用
收藏
页码：67 / 76
页数：10
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