Biochemical and Molecular Characterization of Secreted α-Xylosidase from Aspergillus niger

被引：23
|
作者：
Scott-Craig, John S.
Borrusch, Melissa S.
Banerjee, Goutami
Harvey, Christopher M.
Walton, Jonathan D. [1 ]
机构：
[1] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 50期
关键词：
XYLOGLUCAN OLIGOSACCHARIDES; DEGRADING ENZYMES; CLASSIFICATION; DECONSTRUCTION; PURIFICATION; MIXTURES; PROTEINS; REVEALS; CLONING;
D O I：
10.1074/jbc.M111.307397
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: Microbial secreted alpha-xylosidases are rare. Results: An alpha-xylosidase of Aspergillus niger (AxlA) was purified and expressed in Pichia pastoris. Conclusion: Native and recombinant AxlA act on pNP alpha X and isoprimeverose. Together with beta-glucosidase, AxlA degrades xyloglucan (XG) heptasaccharide. Together with xyloglucanase, beta-galactosidase, and beta-glucosidase, it degrades tamarind XG. Significance: The biochemical function of AxlA is established.
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页码：42848 / 42854
页数：7
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