Biochemical and Molecular Characterization of Secreted α-Xylosidase from Aspergillus niger

被引：23

作者：

Scott-Craig, John S.

Borrusch, Melissa S.

Banerjee, Goutami

Harvey, Christopher M.

Walton, Jonathan D. ^{[1
]}

机构：

[1] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 50期

关键词：

XYLOGLUCAN OLIGOSACCHARIDES; DEGRADING ENZYMES; CLASSIFICATION; DECONSTRUCTION; PURIFICATION; MIXTURES; PROTEINS; REVEALS; CLONING;

D O I：

10.1074/jbc.M111.307397

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Microbial secreted alpha-xylosidases are rare. Results: An alpha-xylosidase of Aspergillus niger (AxlA) was purified and expressed in Pichia pastoris. Conclusion: Native and recombinant AxlA act on pNP alpha X and isoprimeverose. Together with beta-glucosidase, AxlA degrades xyloglucan (XG) heptasaccharide. Together with xyloglucanase, beta-galactosidase, and beta-glucosidase, it degrades tamarind XG. Significance: The biochemical function of AxlA is established.

引用

页码：42848 / 42854

页数：7

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