Electrochemical immunosensor for the detection of atrazine

被引：0
|
作者：
López, MA
Ortega, F
Domínguez, E
Katakis, I
机构：
[1] Univ Alcala de Henares, Facultad Farm, Departamento Quim Analit, E-28871 Alcala De Henares, Spain
[2] Univ Rovira & Virgili, Escola Tecn Super Enginyeria Quim, Dept Enginyieria Quim, E-43006 Tarragona, Spain
来源：
JOURNAL OF MOLECULAR RECOGNITION | 1998年 / 11卷 / 1-6期
关键词：
immunoelectrode; amperometry; atrazine; redox polymers;
D O I：
10.1002/(SICI)1099-1352(199812)11:1/6<178::AID-JMR417>3.0.CO;2-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)(2)Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel, In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl). (C) 1998 John Whey & Sons, Ltd.
引用
收藏
页码：178 / 181
页数：4
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