Monitoring and risk assessment of tetracycline residues in foods of animal origin

A total of 450 samples consisting of red meat, poultry meat, aquatic product and raw milk were collected during winter 2016 and summer 2017. 22.2% (100/450) of collected meat and raw milk samples were found to be contaminated with antibiotic residues in the initial screening using Premi(R)test. According to the enzyme linked immunosorbent assay (ELISA) results, the mean tetracyclines (TCs) concentration of meat samples determined as follows: chicken (155.41 mu g/kg) > turkey (138.68 mu g/kg) > quail (130.7 mu g/kg) > cow (108.92 mu g/kg) > calf (105.18 mu g/kg) > goat (99.4 mu g/kg) > sheep (95.22 mu g/kg) > rainbow trout (35.62 mu g/kg) > shrimp (31.80 mu g/kg). The content of TCs in cow, goat and sheep milk samples were found to be ranged 45.6-163.5 mu g/L, 72.4-101.1 mu g/L and 65.5-98.9 mu g/L, respectively. 5.7% (26/450) of samples had TCs confirmed the ELISA results using high performance liquid chromatography coupled with ultra-violet detection, although the concentration of TCs residues in samples was higher than that of ELISA.