AEBP2 as a transcriptional activator and its role in cell migration

被引:16
|
作者
Kim, Hana [1 ]
Ekram, Muhammad B. [1 ]
Bakshi, Arundhati [1 ]
Kim, Joomyeong [1 ]
机构
[1] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA
关键词
Aebp2; Polycomb Repressive Complex 2; Cell migration; Neural crest cells; EMBRYONIC STEM-CELLS; REGULATORY NETWORK; GENE-EXPRESSION; POLYCOMB; COMPLEX; PRC2; REPRESSION; REGIONS; PROTEIN; GENOME;
D O I
10.1016/j.ygeno.2014.11.007
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aebp2 encodes an evolutionarily conserved zinc finger protein that has not been well studied so far, yet recent studies indicated that this gene is closely associated with the Polycomb Repressive Complex 2 (PRC2). Thus, the current study characterized the basic aspects of this gene, including alternative promoters and protein isoforms. According to the results, Aebp2 is controlled through three alternative promoters, deriving three different transcripts encoding the embryonic (32 kDa) and somatic (52 kDa) forms. Chromatin Immuno-Precipitation (ChIP) experiments revealed that AEBP2 binds to its own promoter as well as the promoters of Jarid2 and Snai2. While the embryonic formacts as a transcriptional repressor for Snai2, the somatic form functions as a transcriptional activator for Jarid2, Aebp2 and Snai2. Cell migration assays also demonstrated that the Aebp2 somatic form has an enhancing activity in cell migration. This is consistent with the functional association of Aebp2 with migratory neural crest cells. These results suggest that the two protein isoforms of AEBP2 may have opposite functions for the PcG target genes, and may play significant roles in cell migration during development. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码:108 / 115
页数:8
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