Characterization of lanthanoid-binding proteins using NMR spectroscopy

被引：5

作者：

Ravera, Enrico ^{[1
,2
,3
]}

Cerofolini, Linda ^{[3
]}

Fragai, Marco ^{[1
,2
,3
]}

Parigi, Giacomo ^{[1
,2
,3
]}

Luchinat, Claudio ^{[1
,2
,3
]}

机构：

[1] Univ Florence, Magnet Resonance Ctr CERM, Sesto Fiorentino, Italy

[2] Univ Florence, Dept Chem Ugo Schiff, Sesto Fiorentino, Italy

[3] Consorzio Interuniv Risonanze Magnet Metalloprot, Sesto Fiorentino, Italy

来源：

RARE-EARTH ELEMENT BIOCHEMISTRY: CHARACTERIZATION AND APPLICATIONS OF LANTHANIDE-BINDING BIOMOLECULES | 2021年 / 651卷

关键词：

X-RAY DATA; PSEUDOCONTACT SHIFTS; PARAMAGNETIC METALLOPROTEINS; BIOLOGICAL MACROMOLECULES; MAGNETIC-SUSCEPTIBILITY; RELAXATION PROCESSES; DISORDERED PROTEINS; NUCLEAR-RELAXATION; XPLOR-NIH; REFINEMENT;

D O I：

10.1016/bs.mie.2021.01.039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The variety of magnetic properties exhibited by paramagnetic lanthanoids provides outstanding information in NMR-based structural biology and therefore can be a very useful tool for characterizing lanthanoid-binding proteins. Because of their dependence on the relative positions of the protein nuclei and of the lanthanoid ion, the paramagnetic restraints (PCS, PRDC and PRE) provide information on structure and dynamics of proteins. In this Chapter, we cover the use of lanthanoids in structural biology including protein sample preparation, NMR experiments and data interpretation.

引用

页码：103 / 137

页数：35

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