Expression, purification and crystallization of a plant type III polyketide synthase that produces diarylheptanoids

被引：5

作者：

Morita, Hiroyuki ^{[2
]}

Wanibuchi, Kiyofumi ^{[2
]}

Kato, Ryohei ^{[1
]}

Sugio, Shigetoshi ^{[1
]}

Abe, Ikuro ^{[2
]}

机构：

[1] Mitsubishi Chem Grp Sci & Technol Res Ctr Inc, Biotechnol Lab, Aoba Ku, Kanagawa 2278502, Japan

[2] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

关键词：

curcuminoid synthases; curcumin synthases; diketide-CoA synthases; polyketide synthases; chalcone synthases;

D O I：

10.1107/S1744309110023572

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Curcuminoid synthase (CUS) from Oryza sativa is a plant-specific type III polyketide synthase that catalyzes the one-pot formation of bisdemethoxycurcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA. Recombinant CUS was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a = 72.7, b = 97.2, c = 126.2 A, alpha = gamma = 90.0, beta = 103.7 degrees. A diffraction data set was collected in-house to 2.5 A resolution.

引用

页码：948 / 950

页数：3

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