A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells

被引：5
|
作者：
Sherman, Jonathan H. [1 ]
Redpath, Gerard T. [2 ]
Redick, Jan A. [3 ]
Purow, Benjamin W. [4 ]
Laws, Edward R. [5 ]
Jane, John A., Jr. [1 ]
Shaffrey, Mark E. [1 ]
Hussaini, Isa M. [2 ]
机构：
[1] Univ Virginia, Dept Neurol Surg, Charlottesville, VA USA
[2] Univ Virginia, Dept Pathol, Charlottesville, VA 22903 USA
[3] Univ Virginia, Sch Med, Core Facil, Charlottesville, VA 22908 USA
[4] Univ Virginia, Dept Neurol, Charlottesville, VA USA
[5] Brigham & Womens Hosp, Dept Neurol Surg, Boston, MA 02115 USA
来源：
JOURNAL OF NEUROSCIENCE METHODS | 2011年 / 198卷 / 01期
关键词：
1,4-Benzoquinone; Immunofluorescence; CD; 133; Glioblastoma stem cells; TUMOR-INITIATING CELLS; PANCREATIC-CANCER; GROWTH; IDENTIFICATION; POPULATIONS; MARKER;
D O I：
10.1016/j.jneumeth.2011.03.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1.4-benzoquinone) for immunoflourescence staining of neurospheres. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：99 / 102
页数：4
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