A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells

被引：5
|
作者：
Sherman, Jonathan H. [1 ]
Redpath, Gerard T. [2 ]
Redick, Jan A. [3 ]
Purow, Benjamin W. [4 ]
Laws, Edward R. [5 ]
Jane, John A., Jr. [1 ]
Shaffrey, Mark E. [1 ]
Hussaini, Isa M. [2 ]
机构：
[1] Univ Virginia, Dept Neurol Surg, Charlottesville, VA USA
[2] Univ Virginia, Dept Pathol, Charlottesville, VA 22903 USA
[3] Univ Virginia, Sch Med, Core Facil, Charlottesville, VA 22908 USA
[4] Univ Virginia, Dept Neurol, Charlottesville, VA USA
[5] Brigham & Womens Hosp, Dept Neurol Surg, Boston, MA 02115 USA
来源：
JOURNAL OF NEUROSCIENCE METHODS | 2011年 / 198卷 / 01期
关键词：
1,4-Benzoquinone; Immunofluorescence; CD; 133; Glioblastoma stem cells; TUMOR-INITIATING CELLS; PANCREATIC-CANCER; GROWTH; IDENTIFICATION; POPULATIONS; MARKER;
D O I：
10.1016/j.jneumeth.2011.03.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1.4-benzoquinone) for immunoflourescence staining of neurospheres. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：99 / 102
页数：4
相关论文
共 50 条
  • [1] CD133-positive stem cells
    Bian, Er-Bao
    Li, Jia
    Zhao, Bing
    JOURNAL OF NEUROSURGERY, 2014, 120 (04) : 1011 - 1012
  • [2] CD133-positive stem cells RESPONSE
    Tamura, Kaoru
    Aoyagi, Masaru
    JOURNAL OF NEUROSURGERY, 2014, 120 (04) : 1012 - 1012
  • [3] Notch pathway blockade inhibits glioblastoma growth by depleting CD133-positive cancer stem cells
    Fan, Xing
    Khaki, Leila
    Koh, Cheryl
    Zhang, Jiangyang
    Li, Yue-Ming
    DiMeco, Francesco
    Vescovi, Angelo
    Eberhart, Charles
    NEURO-ONCOLOGY, 2007, 9 (04) : 594 - 594
  • [4] CD133-Positive Hematopoietic Stem Cells: From Biology to Medicine
    Handgretinger, Rupert
    Kuci, Selim
    PROMININ-1 (CD133): NEW INSIGHTS ON STEM & CANCER STEM CELL BIOLOGY, 2013, 777 : 99 - 111
  • [5] Type 1 collagen as a potential niche component for CD133-positive glioblastoma cells
    Motegi, Hiroaki
    Kamoshima, Yuuta
    Terasaka, Shunsuke
    Kobayashi, Hiroyuki
    Houkin, Kiyohiro
    NEUROPATHOLOGY, 2014, 34 (04) : 378 - 385
  • [6] CD44/CD133-Positive Colorectal Cancer Stem Cells Are Sensitive to Trifluridine
    Tsunekuni, Kenta
    Konno, Masamitsu
    Koseki, Jun
    Asai, Ayumu
    Doki, Yuichiro
    Mori, Masaki
    CANCER SCIENCE, 2018, 109 : 1377 - 1377
  • [7] Functional Differences of miR-125b on the Invasion of Primary Glioblastoma CD133-Negative Cells and CD133-Positive Cells
    Shi, Lei
    Wan, Yi
    Sun, Guan
    Gu, Xiaoyan
    Qian, Chunfa
    Yan, Wei
    Zhang, Shuguang
    Pan, Tianhong
    Wang, Zhimin
    You, Yongping
    NEUROMOLECULAR MEDICINE, 2012, 14 (04) : 303 - 316
  • [8] Comparison of CD133-Positive and CD133-Negative Colon Cancer Cells
    Chao, Celia
    Ives, Kirk L.
    Townsend, Courtney M.
    Hellmich, Mark R.
    GASTROENTEROLOGY, 2009, 136 (05) : A9 - A9
  • [9] CD133-Positive Cells for Cardiac Stem Cell Therapy: Current Status and Outlook
    Donndorf, Peter
    Steinhoff, Gustav
    PROMININ-1 (CD133): NEW INSIGHTS ON STEM & CANCER STEM CELL BIOLOGY, 2013, 777 : 215 - 227
  • [10] Functional Differences of miR-125b on the Invasion of Primary Glioblastoma CD133-Negative Cells and CD133-Positive Cells
    Lei Shi
    Yi Wan
    Guan Sun
    Xiaoyan Gu
    Chunfa Qian
    Wei Yan
    Shuguang Zhang
    Tianhong Pan
    Zhimin Wang
    Yongping You
    NeuroMolecular Medicine, 2012, 14 : 303 - 316
12345