Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

被引：29
|
作者：
Torelli, Riccardo [1 ]
Cacaci, Margherita [1 ]
Papi, Massimiliano [3 ]
Sterbini, Francesco Paroni [1 ]
Martini, Cecilia [1 ]
Posteraro, Brunella [2 ]
Palmieri, Valentina [3 ,4 ]
De Spirito, Marco [3 ]
Sanguinetti, Maurizio [1 ]
Bugli, Francesca [1 ]
机构：
[1] Univ Cattolica SC, Inst Microbiol, Lgo F Vito 1, I-00168 Rome, Italy
[2] Univ Cattolica SC, Inst Hyg, Lgo F Vito 1, I-00168 Rome, Italy
[3] Univ Cattolica SC, Inst Phys, Lgo F Vito 1, I-00168 Rome, Italy
[4] Natl Res Council ISC CNR, Inst Complex Syst, Via Taurini 19, I-00185 Rome, Italy
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2017年 / 158卷
关键词：
Biofilm; Vancomycin; Alginate lyase; Deoxyribonuclease I; E; faecalis; faecium; ALGINATE LYASE; IN-VITRO; ASPERGILLUS-FUMIGATUS; RESISTANCE; THERAPY; DNASE; VANCOMYCIN; BACTERIA;
D O I：
10.1016/j.colsurfb.2017.07.010
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：349 / 355
页数：7
相关论文
共 50 条
  • [41] Genotypic characterization and biofilms of shigatoxigenic E. coli isolated from human clinical cases
    Emilia Caceres, Maria
    Marcelo Zotta, Claudio
    Lavayen, Silvina
    Etcheverria, Analia
    Lia Padola, Nora
    ACTA BIOQUIMICA CLINICA LATINOAMERICANA, 2022, 56 (02): : 171 - 180
  • [42] Diversity of E. faecalis isolated from different clinical specimens by using RAPD- PCR in Hilla Province, Iraq
    Al-hussainy, Shymaa A. G.
    Abdul-Lateef, Lamees A.
    RAWAL MEDICAL JOURNAL, 2023, 48 (04): : 822 - 826
  • [43] Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants
    Gurpreet Kaur
    Tejinder Pal Singh
    Ravinder Kumar Malik
    Arun Bhardwaj
    Sachinandan De
    Journal of Food Science and Technology, 2014, 51 : 233 - 244
  • [44] Detection of poxtA-and optrA-carrying E. faecium isolates in air samples of a Spanish swine farm
    Ruiz-Ripa, Laura
    Fessler, Andrea T.
    Hanke, Dennis
    Sanz, Susana
    Olarte, Carmen
    Eichhorn, Inga
    Schwarz, Stefan
    Torres, Carmen
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2020, 22 : 28 - 31
  • [45] Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants
    Kaur, Gurpreet
    Singh, Tejinder Pal
    Malik, Ravinder Kumar
    Bhardwaj, Arun
    De, Sachinandan
    JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2014, 51 (02): : 233 - 244
  • [46] Genotype–phenotype correlation of fecal Streptococcus regulator (fsr) locus with gelatinase activity and biofilm formation intensity in clinical E. faecalis isolates
    Khaled A. Abdelrahman
    Mona T. Kashef
    Ramy K. Aziz
    Yomna A. Hashem
    Future Journal of Pharmaceutical Sciences, 10
  • [47] Molecular detection of aggregation substance, enterococcal surface protein, and cytolysin genes and in vitro adhesion to urinary catheters of Enterococcus faecalis and E. faecium of clinical origin
    Hallgren, Anita
    Claesson, Carina
    Saeedi, Baharak
    Monstein, Hans-Juerg
    Hanberger, Hakan
    Nilsson, Lennart E.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2009, 299 (05) : 323 - 332
  • [48] Horizontal transfer of antibiotic resistance from Enterococcus faecium of fermented meat origin to clinical isolates of E-faecium and Enterococcus faecalis
    Jahan, Musarrat
    Zhanel, George G.
    Sparling, Richard
    Holley, Richard A.
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2015, 199 : 78 - 85
  • [49] The survey of risk factors of multi drug resistant of E. faecalis isolated from clinical samples
    Moniri, R.
    INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2010, 14 : E343 - E343
  • [50] Combined Sodium Hypochlorite and 940 nm Diode Laser Treatment Against Mature E. Faecalis Biofilms in-vitro
    Castelo-Baz, Pablo
    Martin-Biedma, Benjamin
    Ruiz-Pinon, Manuel
    Rivas-Mundina, Berta
    Bahillo, Jose
    Seoane-Prado, Rafael
    Perez-Estevez, Antonio
    Gude, Francisco
    De Moor, Roeland
    Varela-Patino, Purificacion
    JOURNAL OF LASERS IN MEDICAL SCIENCES, 2012, 3 (03) : 116 - 121
12345