Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

被引：29

作者：

Torelli, Riccardo ^{[1
]}

Cacaci, Margherita ^{[1
]}

Papi, Massimiliano ^{[3
]}

Sterbini, Francesco Paroni ^{[1
]}

Martini, Cecilia ^{[1
]}

Posteraro, Brunella ^{[2
]}

Palmieri, Valentina ^{[3
,4
]}

De Spirito, Marco ^{[3
]}

Sanguinetti, Maurizio ^{[1
]}

Bugli, Francesca ^{[1
]}

机构：

[1] Univ Cattolica SC, Inst Microbiol, Lgo F Vito 1, I-00168 Rome, Italy

[2] Univ Cattolica SC, Inst Hyg, Lgo F Vito 1, I-00168 Rome, Italy

[3] Univ Cattolica SC, Inst Phys, Lgo F Vito 1, I-00168 Rome, Italy

[4] Natl Res Council ISC CNR, Inst Complex Syst, Via Taurini 19, I-00185 Rome, Italy

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2017年 / 158卷

关键词：

Biofilm; Vancomycin; Alginate lyase; Deoxyribonuclease I; E; faecalis; faecium; ALGINATE LYASE; IN-VITRO; ASPERGILLUS-FUMIGATUS; RESISTANCE; THERAPY; DNASE; VANCOMYCIN; BACTERIA;

D O I：

10.1016/j.colsurfb.2017.07.010

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：349 / 355

页数：7

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