Recording Kinetics of Adrenergic Receptor Activation in Live Cells

被引：0

作者：

Vilardaga, Jean-Pierre ^{[1
]}

机构：

[1] Univ Pittsburgh, Sch Med, Dept Pharmacol & Chem Biol, Lab GPCR Biol, Pittsburgh, PA 15260 USA

来源：

ADVANCES IN ADRENERGIC RECEPTOR BIOLOGY | 2011年 / 67卷

关键词：

PROTEIN-COUPLED RECEPTORS; PARATHYROID-HORMONE RECEPTOR; CONFORMATIONAL-CHANGES; MOLECULAR-BASIS; BETA-ARRESTINS; DRUG EFFICACY; LIVING CELLS; BETA(2)-ADRENOCEPTOR; TRAFFICKING; RHODOPSIN;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G protein-coupled receptors (GPCR) biosensors have been recently developed that measure intramolecular conformational changes by recording fluorescence resonance energy transfer (FRET) between fluorescent protein tags introduced at two intracellular sites in the receptor. This technique allows the spatial and temporal recording of agonist (full, partial, and inverse)-induced receptor conformational changes in live cells in real time. This review discusses the kinetics of receptor activation, the direct measurement of ligand efficacy at the level of the receptor, and how ligand efficacy can be modulated by receptor heteromers.

引用

页码：101 / 111

页数：11

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