Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil

被引:6
|
作者
Martins, Thais P. [1 ,2 ]
Souza, Tadeu A. [2 ]
da Silva, Patricia S. [2 ]
Nakasu, Erich Y. T. [2 ]
Melo, Fernando L. [1 ]
Inoue-Nagata, Alice K. [2 ]
Nagata, Tatsuya [1 ]
机构
[1] Univ Brasilia, Dept Mol Biol, Brasilia, DF, Brazil
[2] Embrapa Vegetables, Lab Virol, Brasilia, DF, Brazil
基金
巴西圣保罗研究基金会;
关键词
D O I
10.1007/s00705-021-05220-w
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
During a survey in a tomato field in Luziania (Goias State, Brazil), a single plant with mottling, chlorotic spots, and leaf distortion was found. A new bipartite begomovirus sequence was identified using nanopore sequence technology and confirmed by Sanger sequencing. The highest nucleotide sequence identity match of the DNA-A component (2596 bases) was 81.64% with tomato golden leaf deformation virus (HM357456). Due to the current species demarcation criterion of 91% nucleotide sequence identity for DNA-A, we propose this virus to be a new member of the genus Begomovirus, named "tomato mottle leaf distortion virus".
引用
收藏
页码:3217 / 3220
页数:4
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