Enhanced chemiluminescence: A sensitive analytical system for detection of sweet potato peroxidase

被引：9
|
作者：
Vdovenko, Marina M. [1 ]
Della Ciana, Leopoldo [2 ]
Sakharov, Ivan Y. [1 ,3 ]
机构：
[1] Moscow MV Lomonosov State Univ, Fac Chem, Chem Enzymol Dept, Moscow 119991, Russia
[2] Cyanagen Srl, Bologna, Italy
[3] GV Plekhanov Russian Econ Acad, Div Chem, Moscow, Russia
来源：
BIOTECHNOLOGY JOURNAL | 2010年 / 5卷 / 08期
基金：
俄罗斯基础研究基金会;
关键词：
Chemiluminescence; Enhancer; 4-Morpholinopyridine; Peroxidase 3-(10 '-Phenothiazinyl)propane-1-sulfonate; SOYBEAN PEROXIDASE; HYDROGEN-PEROXIDE; LUMINOL; OXIDATION;
D O I：
10.1002/biot.201000033
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
3-(10'-Phenothiazinyl)propane-l-sulfonate (SPTZ) was shown to be a potent enhancer of anionic sweet potato peroxidase (aSPP)-induced chemiluminescence The optimal conditions for aSPP-catalyzed oxidation of luminol were investigated by varying the concentrations of luminol, hydrogen peroxide, Tris, and SPTZ as well as the pH values of the reaction mixture Addition of 4-morphohnopyridine (MORP) to the reaction mixture markedly increased the light intensity Using SPTZ and MORP together enhanced the effect 265 times The lower detection limit (LDL) of SPP was 009 pM, approximately in 10 times lower than that for the cationic isozyme c of horseradish peroxidase/4-iodophenol system It was shown that aSPP in the presence of SPTZ produced a longer lasting chemiluminescent signal
引用
收藏
页码：886 / 890
页数：5
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