Synergistic effects of FGF-2 and PDGF-BB on angiogenesis and muscle regeneration in rabbit hindlimb ischemia model

被引：56

作者：

Li, Jie ^{[1
,2
,3
]}

Wei, Yuquan ^{[1
,2
]}

Liu, Kang ^{[1
,2
,3
]}

Yuan, Chuang ^{[1
,2
]}

Tang, Yajuan ^{[1
,2
]}

Quan, Qingli ^{[1
,2
,3
]}

Chen, Ping ^{[1
,2
]}

Wang, Wei ^{[1
,2
,3
]}

Hu, Huozhen ^{[1
,2
,3
]}

Yang, Li ^{[1
,2
]}

机构：

[1] Sichuan Univ, State Key Lab Biotherapy, Chengdu, Sichuan, Peoples R China

[2] Sichuan Univ, Ctr Canc, W China Hosp, W China Med Sch, Chengdu, Sichuan, Peoples R China

[3] Sichuan Univ, Coll Life Sci, Lab Med Biol & Cell Biol, Chengdu, Sichuan, Peoples R China

来源：

MICROVASCULAR RESEARCH | 2010年 / 80卷 / 01期

关键词：

Basic fibroblast growth factor; Platelet-derived growth factor; Naked plasmid DNA; Therapeutic angiogenesis; Muscle regeneration; ENDOTHELIAL GROWTH-FACTOR; PERIPHERAL ARTERIAL-DISEASE; SKELETAL-MUSCLE; IN-VIVO; MYOCARDIAL-INFARCTION; GENE DELIVERY; BLOOD-FLOW; VEGF; CELLS; ANGIOPOIETIN-1;

D O I：

10.1016/j.mvr.2009.12.002

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Combinatorial strategy has been used in therapeutic angiogenesis in animal models of peripheral arterial disease (PAD) and coronary artery disease for decades. Previous studies have shown that basic fibroblast growth factor (FGF-2) and platelet-derived growth factor BB (PDGF-BB) proteins together establish functional and stable vascular networks on mouse corneal and also in animal model of hindlimb ischemia. However, the short half life of protein by single injection is not sufficient to achieve effective dosage, repeated and prolonged injection causes systemic toxicity. Here we study the synergistic effects of FGF-2 and PDGF-BB by intramuscular injection of naked plasmid DNA on therapeutic angiogenesis in rabbit model of hindlimb ischemia. We found that transient delivery of FGF-2 and PDGF-BB naked DNA together resulted in greater increases in capillary growth, collateral formation and popliteal blood flow compared with control and single gene delivery. Our data provided novel evidence of beneficial effects of DNA-based FGF-2 and PDFG-BB on muscle repair after ischemic injury. These findings reveal an alternative therapeutic approach in the treatment of ischemic diseases and even in muscular disorders. (C) 2010 Published by Elsevier Inc.

引用

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页码：10 / 17

页数：8

共 43 条

[41] Activation of extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2) by FGF-2 and PDGF-BB in normal human osteoblastic and bone marrow stromal cells: Differences in mobility and in-gel renaturation of ERK1 in human, rat, and mouse osteoblastic cells
Chaudhary, LR
Avioli, LV
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 238 (01) : 134 - 139
[42] Dual gene transfer of fibroblast growth factor-2 and platelet derived growth factor-BB using plasmid deoxyribonucleic acid promotes effective angiogenesis and arteriogenesis in a rodent model of hindlimb ischemia
De Paula, Erich V.
Flores-Nascimento, Mariane Cristina
Arruda, Valder R.
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TRANSLATIONAL RESEARCH, 2009, 153 (05) : 232 - 239
[43] Effects of platelet-rich plasma-containing fragmin/protamine microparticles in enhancing endothelial and smooth muscle cell growth and inducing collateral vessels in a rabbit model of hindlimb ischemia
Fujita, Masanori
Horio, Takuya
Kishimoto, Satoko
Nakamura, Shingo
Takikawa, Megumi
Nakayama, Takefumi
Yamamoto, Yoritsuna
Shimizu, Masafumi
Hattori, Hidemi
Tachibana, Shoichi
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2013, 101B (01) : 36 - 42

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