Role of Mg2+ ions in flavin recognition by RNA aptamer

被引：8

作者：

Sengupta, Abhigyan ^{[1
]}

Gavvala, Krishna ^{[1
]}

Koninti, Raj Kumar ^{[1
]}

Hazra, Partha ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res IISER Pune, Dept Chem, Pune 411008, Maharashtra, India

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2014年 / 140卷

关键词：

Flavins; RNA-aptamer; Molecular recognition; Thermodynamics parameters; ADENINE-DINUCLEOTIDE; REDOX POTENTIALS; PH-DEPENDENCE; BINDING; IDENTIFICATION; HIV-1; DYNAMICS; THERMODYNAMICS; MONONUCLEOTIDE; CONFORMATION;

D O I：

10.1016/j.jphotobiol.2014.08.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of Mg2+ ion in flavin (flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN)) recognition by RNA aptamer has been explored through steady state and time-resolved fluorescence, circular dichroism (CD), thermal melting (TM) and isothermal titration calorimetry (ITC) studies. A strong quenching of flavin emission is detected due to stacking interaction with the nucleobases in the mismatched region of aptamer, and it enhances manifold with increasing Mg2+ concentrations. A comparatively lower binding affinity toward FAD compared to FMN is attributed to the presence of intramolecular 'stack' conformer of FAD, which cannot participate in the intermolecular stacking interactions with the nucleobases. CD and TM studies predict that flavin detection causes structural reformation of RNA aptamer ITC results indicate that flavin detection is thermodynamically feasible and highly enthalpy driven. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：240 / 248

页数：9

共 50 条

[21] The role of Mg2+ in immune cells
Brandao, Katherine
Deason-Towne, Francina
Perraud, Anne-Laure
Schmitz, Carsten
IMMUNOLOGIC RESEARCH, 2013, 55 (1-3) : 261 - 269
[22] The role of Mg2+ in immune cells
Katherine Brandao
Francina Deason-Towne
Anne-Laure Perraud
Carsten Schmitz
Immunologic Research, 2013, 55 : 261 - 269
[23] Role of Mg2+, Sr2+, and F- Ions in Octacalcium Phosphate Crystallization
Tsyganova, A. A.
Golovanova, O. A.
INORGANIC MATERIALS, 2017, 53 (12) : 1261 - 1269
[24] Cyclosporin A tubular effects contribute to nephrotoxicity:: role for Ca2+ and Mg2+ ions
da Costa, MC
de Castro, I
Neto, ALC
Ferreira, AT
Burdmann, EA
Yu, L
NEPHROLOGY DIALYSIS TRANSPLANTATION, 2003, 18 (11) : 2262 - 2268
[25] A Mg2+-responding RNA That Controls the Expression of a Mg2+ Transporter
Groisman, E. A.
Cromie, M. J.
Shi, Y.
Latifi, T.
REGULATORY RNAS, 2006, 71 : 251 - 258
[26] A Mg2+-responding RNA that controls the expression of a Mg2+ transporter
Groisman, E. A.
Cromie, M. J.
Shi, Y.
Latifi, T.
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 2006, 71 : 251 - 258
[27] Nonagermanide Zintl Clusters with Mg2+ Counter Ions
Wallach, Christoph
Klein, Wilhelm
Faessler, Thomas F.
ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE, 2022, 648 (19):
[28] INTRACELLULAR MG2+ ([MG2+](1)), SUBCELLULAR COMPARTMENTATION OF [MG2+](I) AND ITS REGULATION BY EXTRACELLULAR MG2+ ([MG2+](O)) IN YEAST
ZHANG, A
CHENG, TPO
WU, XY
ALTURA, BT
ALTURA, BM
FASEB JOURNAL, 1995, 9 (06): : A1445 - A1445
[29] Mg2+ ions: do they bind to nucleobase nitrogens?
Leonarski, Filip
D'Ascenzo, Luigi
Auffinger, Pascal
NUCLEIC ACIDS RESEARCH, 2017, 45 (02) : 987 - 1004
[30] METABOLISM AND PHYSICOCHEMICAL STATE OF MG2+ IONS IN FUNGI
OKOROKOV, LA
LICHKO, LP
KADOMTSEVA, VM
KHOLODENKO, VP
KULAEV, IS
MICROBIOLOGY, 1974, 43 (03) : 347 - 351

← 1 2 3 4 5 →