Preparation of smooth siloxane surfaces for AFM visualization of immobilized biomolecules

被引：23
|
作者：
Tätte, T
Saal, K
Kink, I
Kurg, A
Lohmus, R
Mäeorg, U
Rahi, M
Rinken, A
Lohmus, A
机构：
[1] Univ Tartu, Inst Phys, EE-51014 Tartu, Estonia
[2] Univ Tartu, Inst Organ & Bioorgan Chem, EE-51014 Tartu, Estonia
[3] Estonian Bioctr, Inst Mol & Cell Biol, EE-51010 Tartu, Estonia
[4] Estonian Agr Univ, Inst Zool & Bot, Tartu, Estonia
来源：
SURFACE SCIENCE | 2003年 / 532卷
关键词：
atomic force microscopy; surface structure; morphology; roughness; and topography; Mica; Silane; biological molecules nucleic acids;
D O I：
10.1016/S0039-6028(03)00486-2
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Development of scanning probe microscopy (SPM) techniques during past decade has made possible to study bioprocesses at a molecular level. Common protocols for immobilization of biomolecules for SPM studies are based on their adsorption to alkylsiloxane monolayers on hydroxylated surfaces. The same procedure is also widely used for DNA chip and biosensor fabrication. For SPM studies of immobilized biomolecules smooth carrier surfaces are mandatory. Several studies refer to the lack of reproducibility in formation of smooth silanized surfaces. In this work a new method based on carrier treatment with linearly polymerized 3-aminopropyltrimethoxysilane for preparation of reproducibly smooth silanized surfaces is proposed. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：1085 / 1091
页数：7
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