Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus

被引：47
|
作者：
Stratford, R [1 ]
Douce, G [1 ]
Zhang-Barber, L [1 ]
Fairweather, N [1 ]
Eskola, J [1 ]
Dougan, G [1 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, England
来源：
VACCINE | 2000年 / 19卷 / 7-8期
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
DNA vaccines; tetanus; codon usage; murine model;
D O I：
10.1016/S0264-410X(00)00246-2
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/ stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：810 / 815
页数：6
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