Functional silver nanozymes regulate cell inflammatory cytokines expression in mouse macrophages

The increased biomedical applications of enzyme-mimicking nanoparticles, also known as 'nanozymes' has necessitated their controlled design in terms of physicochemical properties, and reduced toxicity. In this context, numerous approaches have been established to manufacture nanozymes using eco-friendly routes, however, controlled surfaces and suitable functionalization of nanozymes need much attention to utilize their full po-tential. Here, serine synthesised silver (Ag) nanozymes have been fabricated, and sequentially surface func-tionalized with isonicotinic acid hydrazide (isoniazid), streptomycin, and phosphomolybdic acid to control their nanozyme-mimicking nature, and impart biological capabilities. Post-physicochemical characterization, all these functional Ag-centred nanozymes were explored for their inherent peroxidase enzyme-like behavior, and haemcompatibility. The cell viability and proliferation assessments towards mouse RAW 264.7 macrophages confirmed the cytocompatible nature of Ag nanozymes. Moreover, these nanozymes show anti-inflammatory potential by regulating the key inflammatory cytokines IL-6, IL-beta, and TNF-alpha.