Role of the C-terminal cysteines in virus-like particle formation and oligomerization of the hepatitis E virus ORF2 truncated proteins

被引：7
|
作者：
Liu, Zhenzhen [1 ,2 ]
Behloul, Nouredine [1 ]
Baha, Sarra [2 ]
Wei, Wenjuan [2 ]
Tao, Wanru [3 ]
Zhang, Tingying [1 ]
Li, Wei [1 ,3 ]
Shi, Ruihua [2 ]
Meng, Jihong [1 ,2 ]
机构：
[1] Shanghai Univ Med & Hlth Sci, Coll Basic Med, Shanghai 201318, Peoples R China
[2] Southeast Univ, Zhongda Hosp, Dept Gastroenterol, Nanjing 210009, Jiangsu, Peoples R China
[3] Second Mil Med Univ, Lab Nano Biomed, Shanghai 200433, Peoples R China
来源：
VIROLOGY | 2020年 / 544卷
基金：
中国国家自然科学基金;
关键词：
Hepatitis E virus; Virus-like particle; Capsid assembly; Disulfide bond; CAPSID PROTEIN; DISULFIDE BONDS; IMMUNOMAGNETIC SEPARATION; STRUCTURAL BASIS; NON-A; NEUTRALIZATION; DIMERIZATION; PREDICTION;
D O I：
10.1016/j.virol.2020.01.011
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The hepatitis E virus (HEV) ORF2 truncated recombinant proteins can self-assemble into virus-like particles (VLPs) and were used as models to investigate the HEV capsid assembly. However, the structural function of the ORF2 C-terminal domain (C52aa from aa 608 to aa 660) remains unclear. Herein, by analyzing a set of ORF2 truncated proteins expressed in Escherichia coli, we found that the highly conserved C-terminal cysteines play a crucial role in the oligomerization of the truncated ORF2 proteins and in their assembly into VLPs, through the formation of dimer-dimer disulfide bonds; and the treatment of native HEV particles with dithiothreitol (DTT) induced the disassembly of the viral capsid, suggesting that the disulfide bonding is required for stabilizing the native HEV capsid. The present study sheds light on the structural role of the C-terminal region of the HEV capsid protein and contributes to the full understating of the viral capsid assembly process.
引用
收藏
页码：1 / 11
页数：11
相关论文
共 50 条
  • [31] Virus-like particle-based vaccines against hepatitis C virus infection
    Bellier, Bertrand
    Klatzmann, David
    EXPERT REVIEW OF VACCINES, 2013, 12 (02) : 143 - 154
  • [32] A C-terminal truncated hepatitis C virus core protein variant assembles in vitro into virus-like particles in the absence of structured nucleic acids (vol 334, pg 901, 2005)
    Acosta-Rivero, N
    Rodriguez, A
    Mussachio, A
    Poutou, J
    Falcon, V
    Torres, D
    Aguilar, JC
    Linares, M
    Alonso, M
    Perez, A
    Menendez, I
    Morales-Grillo, J
    Marquez, G
    Dueñas-Carrera, S
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 337 (01) : 410 - 410
  • [33] Prediction of promiscuous epitopes in ORF2 of Hepatitis E virus: an In-Silico approach
    Samavia, Noor
    Fahed, Parvaiz
    Yasir, Waheed
    Tasneem, Anwar
    Syeda, Nasreen
    AFRICAN HEALTH SCIENCES, 2022, 22 (03) : 626 - 639
  • [34] The full-length and N-terminal deletion of ORF2 protein of hepatitis E virus can dimerize
    Tyagi, S
    Jameel, S
    Lal, SK
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 286 (01) : 214 - 221
  • [35] Hepatitis E virus lifecycle and identification of 3 forms of the ORF2 capsid protein
    Montpellier, C.
    Wychowski, C.
    Sayed, I. M.
    Meunier, J. -C.
    Saliou, J. -M.
    Ankavay, M.
    Bull, A.
    Pillez, A.
    Abravanel, F.
    Helle, F.
    Brochot, E.
    Drobecq, H.
    Farhat, R.
    Aliouat-Denis, C. -M.
    Haddad, J. G.
    Izopet, J.
    Meuleman, P.
    Goffard, A.
    Dubuisson, J.
    Cocquerel, L.
    JOURNAL OF HEPATOLOGY, 2018, 68 : S781 - S782
  • [36] Origin, antigenicity, and function of a secreted form of ORF2 in hepatitis E virus infection
    Yin, Xin
    Ying, Dong
    Lhomme, Sebastien
    Tang, Zimin
    Walker, Christopher M.
    Xia, Ningshao
    Zheng, Zizheng
    Feng, Zongdi
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (18) : 4773 - 4778
  • [37] Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein
    Ankavay, Maliki
    Da Silva, Nathalie
    Pollan, Angela
    Oechslin, Noemie
    Dinkelborg, Katja
    Behrendt, Patrick
    Moradpour, Darius
    Gouttenoire, Jerome
    JHEP REPORTS, 2025, 7 (03)
  • [38] The ORF2 protein of hepatitis E virus binds the 5′ region of viral RNA
    Surjit, M
    Jameel, S
    Lal, SK
    JOURNAL OF VIROLOGY, 2004, 78 (01) : 320 - 328
  • [39] An essential RNA element resides in a central region of hepatitis E virus ORF2
    Emerson, Suzanne U.
    Nguyen, Hanh T.
    Torian, Udana
    Mather, Karly
    Firth, Andrew E.
    JOURNAL OF GENERAL VIROLOGY, 2013, 94 : 1468 - 1476
  • [40] Structure of the hepatitis E virus-like particle suggests mechanisms for virus assembly and receptor binding
    Guu, Tom S. Y.
    Liu, Zheng
    Ye, Qiaozhen
    Mata, Douglas A.
    Li, Kunpeng
    Yin, Changcheng
    Zhang, Jingqiang
    Tao, Yizhi Jane
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (31) : 12992 - 12997
12345