DGUOK-AS1 promotes cervical squamous cell carcinoma progression by suppressing miR-499a-5p that targets SPRR1B in vitro

被引:12
|
作者
Song, Yan [1 ]
Pan, Hongjuan [1 ]
Yang, Ling [2 ]
Fan, Yan [1 ]
Zhang, Hui [1 ]
Pan, Meichen [1 ]
Zhang, Yali [3 ]
机构
[1] Taikang Tongji Wuhan Hosp, Dept Obstet, Wuhan 430050, Peoples R China
[2] Shenzhen Baoan Shiyan Peoples Hosp, Dept Gynaecol, Shenzhen 518108, Peoples R China
[3] Shenzhen Longhua Maternal & Child Hlth Hosp, Dept Gynaecol, 68 Dalanghuawang Rd, Shenzhen 518110, Peoples R China
关键词
DGUOK-AS1; miR-499a-5p; SPRR1B; CESC; CANCER CELLS; MIGRATION;
D O I
10.1016/j.bbrc.2021.11.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Purpose: Cervical squamous cell carcinoma (CESC) is the most common cancer type of cervical cancer, which threatens women's life seriously. LncRNA DGUOK-AS1has been reported to promote the biologic processes of CESC. We aim to figure out the role of DGUOK-AS1-miR-499a-5p-SPRR1B axis in modulating the CESC progression in vitro. Methods: The levels of DGUOK-AS1, miR-499a-5p, and SPRR1B in CESC tissues and cells were examined by RT-qPCR. The interaction of DGUOK-AS1-miR-499a-5p-SPRR1B was verified by luciferase assay. Inhibition of DGUOK-AS1, miR-499a-5p, and SPRR1B was applied for exploring the biological function based on detection of cell viability, proliferation, migration, and apoptosis in CESC SiHa and HeLa cells. Results: DGUOK-AS1 and SPRR1B expressions were obviously elevated, whereas the expression of miR499a-5p was reduced in both CESC tissues and cells. Silencing of DGUOK-AS1 attenuated cell growth and boosted apoptosis of CESC cells. Notably, DGUOK-AS1 inhibited miR-499a-5p to release SPRR1B, which significantly accelerated the development of CESC. Conclusion: DGUOK-AS1sponging miR-499a-5p facilitated CESC cells progression by releasing SPRR1B in vitro. It provides a new sight for the treatment of CESC patients involving DGUOK-AS1-miR-499a-5pSPRR1B. (c) 2021 Published by Elsevier Inc.
引用
收藏
页码:177 / 184
页数:8
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