Diacylglycerol acyltransferase in maturing sunflower seeds

被引：0
|
作者：
Triki, S
Ben Hamida, J
Mazliak, P
机构：
[1] INRST, Lab Ameliorat & Adaptat Plantes, Hammam Lif, Tunisia
[2] Fac Sci Tunis, Biochim Lab, Tunis 1060, Tunisia
[3] Univ Paris 06, Lab Physiol Cellulaire & Mol, F-75252 Paris, France
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2000年 / 28卷
关键词：
Helianthus annuus; microsome; triacylglycerol synthesis;
D O I：
10.1042/0300-5127:0280689
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Developing sunflower seeds exhibit a high diacylglycerol acyltransferase (DAGAT, EC 2.3.1.20) activity. The distribution of the enzyme has been studied in subcellular fractions prepared by differential centrifugation of seed homogenate. Its activity was characterized using [1-C-14]oleoyl-CoA and diolein dispersed in Tween 20. Some properties of the microsomal fraction of DAGAT were investigated. Hyperbolic kinetics were observed, the apparent K-m was 60 muM and the specific activity of the reaction 15 pmol/min/mg of protein. Addition of BSA (0.1 %) stimulated oleate incorporation, which was not dependent on the presence of exogenous diacylglycerol. Detergents which might solubilize DAGAT, Triton X-100 and CHAPS, were tested for enzyme inhibition, and CHAPS was found to be the least denaturing.
引用
收藏
页码：689 / 692
页数：4
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