Marisediminitalea mangrovi gen. nov., sp. nov., isolated from marine mangrove sediment, and reclassification of Aestuariibacter aggregatus as Marisediminitalea aggregata comb. nov.

Strain GS-14(T) was isolated from a mangrove sediment sample collected at Beilun Estuary National Nature Reserve, Guangxi Province, PR China. Cells were Gram-stain-negative, strictly aerobic and rod-shaped with a polar flagellum. Optimal growth occurred in the presence of 3-6% (w/v) NaCl, at pH 6-8 and at a temperature of 37 degrees C. The predominant polar lipids were phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone 8 (Q-8) was the sole respiratory quinone. The major fatty acids (>10% of the total fatty acids) were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and C-16:0. The DNA G+C content was 47.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GS-14(T) had the highest sequence similarity to Aestuariibacter aggregatus WH169(T) (96.63%), Aliiglaciecola coringensis AK49(T) (96.56%) and Alteromonas lipolytica JW12(T) (96.22%). In addition, the OrthoANIu value and dDDH values calculated from the genomes of strain GS-14(T) and A. aggregatus WH169(T) were 79.5 and 21.9%, respectively. Based on the polyphasic taxonomic results, strain GS-14(T) is considered to represent a novel species in a new genus, for which the name Marisediminitalea mangrovi gen. nov., sp. nov. is proposed. The type strain of Marisediminitalea mangrovi is GS-14(T) (=KCTC 72401(T)=MCCC 1K03622(T)). Because Aestuariibacter aggregatus WH169(T) clustered with strain GS-14(T) in the phylogenetic trees and was clearly separated from the two species within the genus Aestuariibacter, it is reclassified as a member of the genus Marisediminitalea as Marisediminitalea aggregata comb. nov. (type strain WH169(T)=CGMCC 1.8995(T)=LMG 25283(T)). The type species of the genus Marisediminitalea is Marisediminitalea aggregata gen. nov., comb. nov.