Dexamethasone increases pigment epithelium-derived factor in perfused human eyes

被引：8
|
作者：
Perruccio, Elizabeth M. [2 ]
Rowlette, Laura Leigh S. [3 ]
Comes, Nuria [1 ]
Locatelli-Hoops, Silvia [2 ]
Notari, Luigi [2 ]
Becerra, S. Patricia [2 ]
Borras, Teresa [1 ]
机构：
[1] Univ N Carolina, Dept Ophthalmol, Chapel Hill, NC 27599 USA
[2] NEI, Sect Prot Struct & Funct, Lab Retinal Cell & Mol Biol, NIH, Bethesda, MD USA
[3] Duke Univ, Med Ctr, Durham, NC USA
来源：
CURRENT EYE RESEARCH | 2008年 / 33卷 / 5-6期
关键词：
DEX; human; PEDF; perfused anterior segment; trabecular meshwork;
D O I：
10.1080/02713680802110208
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Purpose: To investigate the effects of dexamethasone (DEX) on pigment epithelium-derived factor (PEDF) cDNA and secreted protein in human trabecular meshwork (TM). Methods: Anterior segment organ cultures were perfused with 0.1 mu M DEX (OD) and vehicle (OS). Primary human TM cells (HTM) were treated with DEX under similar conditions. PEDF mRNA and secreted PEDF protein were quantitated by RT-PCR and Western blot. Results: PEDF mRNA and secreted PEDF protein levels were significantly higher in DEX over vehicle-treated cultures. In contrast, DEX decreased the activity of a 92-kDa gelatinolytic zymogen in organ culture effluents. Conclusion: DEX action in the human TM might include a PEDF-mediating pathway.
引用
收藏
页码：507 / 515
页数：9
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