Bone Morphogenetic Protein-2 Signaling in the Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells Induced by Pulsed Electromagnetic Fields
Pulsed electromagnetic fields (PEMFs) are clinically used with beneficial effects in the treatment of bone fracture healing. This is due to PEMF ability to favor the osteogenic differentiation of mesenchymal stem cells (MSCs). Previous studies suggest that PEMFs enhance the osteogenic activity of bone morphogenetic protein-2 (BMP2) which is used in various therapeutic interventions. This study investigated the molecular events associated to the synergistic activity of PEMFs and BMP2 on osteogenic differentiation. To this aim, human MSCs (hMSCs) were exposed to PEMFs (75 Hz, 1.5 mT) in combination with BMP2, upon detection of the minimal dose able to induce differentiation. Changes in the expression of BMP signaling pathway genes including receptors and ligands, as well as in the phosphorylation of BMP downstream signaling proteins, such as SMAD1/5/8 and MAPK, were analyzed. Results showed the synergistic activity of PEMFs and BMP2 on osteogenic differentiation transcription factors and markers. The PEMF effects were associated to the increase in BMP2, BMP6, and BMP type I receptor gene expression, as well as SMAD1/5/8 and p38 MAPK activation. These results increase knowledge concerning the molecular events involved in PEMF stimulation showing that PEMFs favor hMSCs osteogenic differentiation by the modulation of BMP signaling components.
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Huazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R ChinaHuazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R China
Tian Hongtao
Yang Shuhua
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Huazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R ChinaHuazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R China
Yang Shuhua
Xu Liang
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Huazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R ChinaHuazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R China
Xu Liang
Zhang Yukun
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Huazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R ChinaHuazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R China
Zhang Yukun
Xu Weihua
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Huazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R ChinaHuazhong Univ Sci & Technol, Union Hosp, Dept Orthoped, Tongji Med Coll, Wuhan 430022, Peoples R China
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Dongguk Univ, Dept Med Biotechnol, Seoul 100715, South KoreaDongguk Univ, Dept Med Biotechnol, Seoul 100715, South Korea
Kim, Min-Ok
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Jung, Hyun
Kim, Soo-Chan
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Hankyong Natl Univ, Grad Sch Bio & Informat Technol, Anseong 456749, Kyonggi Do, South KoreaDongguk Univ, Dept Med Biotechnol, Seoul 100715, South Korea
Kim, Soo-Chan
Park, Jung-Keug
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Dongguk Univ, Dept Med Biotechnol, Seoul 100715, South KoreaDongguk Univ, Dept Med Biotechnol, Seoul 100715, South Korea
Park, Jung-Keug
Seo, Young-Kwon
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Dongguk Univ, Dept Med Biotechnol, Seoul 100715, South KoreaDongguk Univ, Dept Med Biotechnol, Seoul 100715, South Korea
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Seoul Natl Univ, Dept Oral & Maxillofacial Surg, Dent Res Inst, Seoul Natl Univ Dent Hosp,Sch Dent,BK Plus 21, Seoul 110768, South KoreaSeoul Natl Univ, Dept Oral & Maxillofacial Surg, Dent Res Inst, Seoul Natl Univ Dent Hosp,Sch Dent,BK Plus 21, Seoul 110768, South Korea
Yang, Hoon Joo
Kim, Ri Youn
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Seoul Natl Univ, Dept Maxillofacial Cell & Dev Biol, Dent Res Inst, Sch Dent,BK Plus 21, Seoul 110768, South KoreaSeoul Natl Univ, Dept Oral & Maxillofacial Surg, Dent Res Inst, Seoul Natl Univ Dent Hosp,Sch Dent,BK Plus 21, Seoul 110768, South Korea
Kim, Ri Youn
Hwang, Soon Jung
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Seoul Natl Univ, Dept Oral & Maxillofacial Surg, Dent Res Inst, Seoul Natl Univ Dent Hosp,Sch Dent,BK Plus 21, Seoul 110768, South Korea
Seoul Natl Univ, Dept Maxillofacial Cell & Dev Biol, Dent Res Inst, Sch Dent,BK Plus 21, Seoul 110768, South KoreaSeoul Natl Univ, Dept Oral & Maxillofacial Surg, Dent Res Inst, Seoul Natl Univ Dent Hosp,Sch Dent,BK Plus 21, Seoul 110768, South Korea