共 22 条
Effect of polysaccharide from cultured Cordyceps sinensis on immune function and anti-oxidation activity of mice exposed to 60Co
被引:115
|作者:
Zhang, Jiali
[1
]
Yu, Yongchao
[1
]
Zhang, Zhaofeng
[1
]
Ding, Ye
[1
]
Dai, Xiaogian
[1
]
Li, Yong
[1
]
机构:
[1] Peking Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Beijing 100191, Peoples R China
关键词:
Cordyceps sinensis;
Immunomodulator;
Anti-oxidation;
HUMAN ENDOTHELIAL-CELLS;
TUMOR-BEARING MICE;
GENE-EXPRESSION;
IL-8;
PRODUCTION;
IN-VITRO;
RADIATION;
CYTOKINES;
MEDICINE;
MACROPHAGES;
LYMPHOCYTES;
D O I:
10.1016/j.intimp.2011.09.019
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
Aim of the study: The present study was designed to investigate the molecular weight (MW), chemical composition and effect of polysaccharide (CS-PS), from the fruiting bodies of cultured Cordyceps sinensis, on immune function and anti-oxidation activity of BALB/c mice exposed to Co-60. Materials and methods: The MW of CS-PS was determined by gel-filtration. The chemical composition of CS-PS was tested by using gas chromatography-mass spectrophotometer (GC-MS). Mice were administered CS-PS with doses of 50, 100 or 200 mg/kg body weight, then exposed to Co-60. The normal control group and irradiated control group were also used. Four days later, lymphocyte proliferation, activity of macrophage phagocytosis, delayed type hypersensitivity (DTH), concentration of malondialdehyde (MDA), total-superoxide dismutase (SOD) enzyme activity, and cytokine expression in serum from the mice were tested. Results: The average molecular weight of CP-PS was 12 kD. The polysaccharide was composed of mannose, rhamnose, arabinose, xylose, glucose and galactose. Lymphocyte proliferation, the activity of macrophage phagocytosis, DTH and total-SOD enzyme activity in the CS-PS groups were significantly enhanced compared to the irradiated control group. Lipid peroxidation level was significantly reduced in the CS-PS groups compared to the irradiated control group. Levels of cytokine IL-4, IL-5 and IL-17 are also affected in the CS-PS groups compared to the irradiated control group. Conclusions: CS-PS, a heteropolysaccharide, enhances immunity activity in mice treated by ionizing radiation, through reducing oxidative injury and modulating the secretion of cytokine IL-4, IL-5 and IL-17. (C) 2011 Elsevier B.V. All rights reserved.
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页码:2251 / 2257
页数:7
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