A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins

被引：23
|
作者：
Mehto, P
Ankelo, M
Hinkkanen, A
Mikhailov, A
Eriksson, JE
Spoof, L
Meriluoto, J
机构：
[1] Abo Akad Univ, Dept Biochem & Pharm, Turku 20521, Finland
[2] Turku Ctr Biotechnol, Turku 20521, Finland
[3] Univ Turku, Dept Biol, Turku 20014, Finland
来源：
TOXICON | 2001年 / 39卷 / 06期
关键词：
blue-green algae; cyanobacteria; toxins; microcystins; time-resolved fluoroimmunoassay;
D O I：
10.1016/S0041-0101(00)00215-4
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1 mug/l. The derection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：831 / 836
页数：6
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