A novel oestrogen-regulated gene in human breast cancer cells identified by differential display

被引：4

作者：

Stephen, R ^{[1
]}

Corcoran, D ^{[1
]}

Darbre, PD ^{[1
]}

机构：

[1] Univ Reading, Sch Anim & Microbial Sci, Div Cell & Mol Biol, Reading RG6 6AJ, Berks, England

来源：

JOURNAL OF MOLECULAR ENDOCRINOLOGY | 1998年 / 20卷 / 03期

关键词：

D O I：

10.1677/jme.0.0200375

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Screening by differential display of oestrogen-sensitive MCF7 human breast cancer cells grown in the short-term (6 days) and long-term (70 weeks) absence of oestrogen has led to the identification of a new oestrogen-regulated mRNA. The cDNA isolated by differential display has 100% homology from nucleotides 615 to 859 of the published sequence for the mRNA for human megakaryocyte CD63 antigen but has a 3' tail extended by 23 nucleotides. Northern blotting has confirmed that this mRNA is regulated by oestrogen in both MCF7 and T47D human breast cancer cell lines.

引用

页码：375 / 380

页数：6

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