Characterization of the surface morphology of durum wheat starch granules using atomic force microscopy

被引：17

作者：

Neethikajan, S. ^{[1
]}

Thomson, D. J. ^{[2
]}

Jayas, D. S. ^{[1
]}

White, N. D. G. ^{[3
]}

机构：

[1] Univ Manitoba, Canadian Wheat Board Ctr Grain Storage Res, Dept Biosyst Engn, Winnipeg, MB R3T 5V6, Canada

[2] Univ Manitoba, Dept Elect & Comp Engn, Winnipeg, MB R3T 5V6, Canada

[3] Agr & Agri Food Canada, Ctr Ceram Res, Winnipeg, MB R3T 2M9, Canada

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2008年 / 71卷 / 02期

关键词：

AFM; growth rings; wheat starch; vitreous; nonvitreous;

D O I：

10.1002/jemt.20534

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Knowledge of the structure and properties of microscopic surfaces of durum wheat starch granules is essential for understanding the functional and physico-chemical properties. The nanoscale surface undulations on the starch granules inside durum wheat macroscopically influence the milling properties. The objective of this study was to visualize the surface morphology and the size of starch grains of vitreous and nonvitreous durum wheat kernels using atomic force microscopy. The distribution of starch granules in the vitreous and nonvitreous durum wheat starch samples were examined and compared. The results of our study confirm the 'blocklet' model of the ultrastructure of the starch granule surface. Image contrast enhancement using UV/ozone treatment of microtomed starch samples improved the imaging of growth rings on the starch samples. The observation of growth rings in the nonvitreous starch granule surfaces indicates that amylopectin is more common than amylose in nonvitreous starch when compared with vitreous starch.

引用

页码：125 / 132

页数：8

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