Validation of reference genes for RT-qPCR analysis in Herbaspirillum seropedicae

被引：8
|
作者：
Villarinho Pessoa, Daniella Duarte [1 ,2 ]
Vidal, Marcia Soares [2 ]
Baldani, Jose Ivo [2 ]
Simoes-Araujo, Jean Luiz [2 ]
机构：
[1] Univ Fed Rio de Janeiro, CCS, Programa Posgrad Biotecnol Vegetal, Campus Ilha do Fundao,Av Carlos Chagas Filho 373, BR-21941590 Rio De Janeiro, RJ, Brazil
[2] EMBRAPA, Ctr Nacl Pesquisa Agrobiol, CNPAB, Lab Genet & Bioquim,Embrapa Agrobiol, BR 465,Km 7 S-N,Pavilhao Johanna Dobereiner, BR-23890000 Seropedica, RJ, Brazil
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2016年 / 127卷
关键词：
Expression stability; Housekeeping genes; qPCR internal control; Herbaspirillum seropedicae; Carbon source; MESSENGER-RNA; EXPRESSION; GLUCONACETOBACTER;
D O I：
10.1016/j.mimet.2016.06.011
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The RT-qPCR technique needs a validated set of reference genes for ensuring the consistency of the results from the gene expression. Expression stabilities for 9 genes from Herbaspirillum seropedicae, strain HRC54, grown with different carbon sources were calculated using geNorm and NormFinder, and the gene rpoA showed the best stability values. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：193 / 196
页数：4
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